@article {6293,
	title = {Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens.},
	journal = {Cell},
	volume = {151},
	year = {2012},
	month = {2012 Dec 7},
	pages = {1370-85},
	abstract = {<p>Optical imaging of the dynamics of living specimens involves tradeoffs between spatial resolution, temporal resolution, and phototoxicity, made more difficult in three dimensions. Here, however, we report that rapid three-dimensional (3D) dynamics can be studied beyond the diffraction limit in thick or densely fluorescent living specimens over many time points by combining ultrathin planar illumination\&nbsp;produced by scanned Bessel beams with super-resolution structured illumination microscopy. We demonstrate in\&nbsp;vivo karyotyping of chromosomes during mitosis and identify different dynamics for the actin cytoskeleton at the dorsal and ventral surfaces of fibroblasts. Compared to spinning disk confocal microscopy, we\&nbsp;demonstrate substantially reduced photodamage when imaging rapid morphological changes in D.\&nbsp;discoideum cells, as well as improved contrast and resolution at depth within developing C.\&nbsp;elegans embryos. Bessel beam structured plane illumination thus promises new insights into complex biological phenomena that require 4D subcellular spatiotemporal detail in either a single or multicellular context.</p>
},
	keywords = {Animals, Brain, Caenorhabditis elegans, Cell Line, Dermatitis, Dictyostelium, Drosophila melanogaster, Fibroblasts, Fluorescence, Humans, Imaging, Karyotyping, Larva, Microscopy, Mitosis, Optical Imaging, Phototoxic, Three-Dimensional, Tumor},
	issn = {1097-4172},
	doi = {10.1016/j.cell.2012.10.008},
	author = {Gao, Liang and Shao, Lin and Higgins, Christopher D and Poulton, John S and Peifer, Mark and Davidson, Michael W and Wu, Xufeng and Goldstein, Bob and Betzig, Eric}
}
@article {4598,
	title = {Triggering a cell shape change by exploiting preexisting actomyosin contractions.},
	journal = {Science},
	volume = {335},
	year = {2012},
	month = {2012 Mar 9},
	pages = {1232-5},
	abstract = {<p>Apical constriction changes cell shapes, driving critical morphogenetic events, including gastrulation in diverse organisms and neural tube closure in vertebrates. Apical constriction is thought to be triggered by contraction of apical actomyosin networks. We found that apical actomyosin contractions began before cell shape changes in both Caenorhabitis elegans and Drosophila. In C. elegans, actomyosin networks were initially dynamic, contracting and generating cortical tension without substantial shrinking of apical surfaces. Apical cell-cell contact zones and actomyosin only later moved increasingly in concert, with no detectable change in actomyosin dynamics or cortical tension. Thus, apical constriction appears to be triggered not by a change in cortical tension, but by dynamic linking of apical cell-cell contact zones to an already contractile apical cortex.</p>
},
	issn = {1095-9203},
	doi = {10.1126/science.1217869},
	author = {Roh-Johnson, Minna and Shemer, Gidi and Higgins, Christopher D and McClellan, Joseph H and Werts, Adam D and Tulu, U Serdar and Gao, Liang and Betzig, Eric and Kiehart, Daniel P and Goldstein, Bob}
}
