@article {6505,
	title = {Betamax: towards optimal sampling strategies for high-throughput screens.},
	journal = {Journal of Computational Biology: A Journal of Computational Molecular Cell Biology},
	volume = {19},
	year = {2012},
	month = {2012 Jun},
	pages = {776-84},
	abstract = {<p>Sample size is a critical component in the design of any high-throughput genetic screening approach. Sample size determination from assumptions or limited data at the planning stages, though standard practice, may at times be unreliable because of the difficulty of a priori modeling of effect sizes and variance. Methods to update the sample size estimate during the course of the study could improve statistical power. In this article, we introduce an approach to estimate the power and update it continuously during the screen. We use this estimate to decide where to sample next to achieve maximum overall statistical power. Finally, in simulations, we demonstrate significant gains in study recall over the naive strategy of equal sample sizes while maintaining the same total number of samples.</p>
},
	keywords = {Algorithms, Computer Simulation, Genetic Testing, High-Throughput Screening Assays, Humans, Models, Research Design, Sample Size, Statistical},
	issn = {1557-8666},
	doi = {10.1089/cmb.2012.0036},
	author = {Grover, Dhruv and Nunez-Iglesias, Juan}
}
@article {5237,
	title = {Hydrogen peroxide stimulates activity and alters behavior in Drosophila melanogaster.},
	journal = {PLoS One},
	volume = {4},
	year = {2009},
	month = {2009 Oct 28},
	pages = {e7580},
	abstract = {<p>Circadian rhythms in animals are regulated at the level of individual cells and by systemic signaling to coordinate the activities of multiple tissues. The circadian pacemakers have several physiological outputs, including daily locomotor rhythms. Several redox-active compounds have been found to function in regulation of circadian rhythms in cells, however, how particular compounds might be involved in regulating specific animal behaviors remains largely unknown. Here the effects of hydrogen peroxide on Drosophila movement were analyzed using a recently developed three-dimensional real-time multiple fly tracking assay. Both hydrogen peroxide feeding and direct injection of hydrogen peroxide caused increased adult fly locomotor activity. Continuous treatment with hydrogen peroxide also suppressed daily locomotor rhythms. Conditional over-expression of the hydrogen peroxide-producing enzyme superoxide dismutase (SOD) also increased fly activity and altered the patterns of locomotor activity across days and weeks. The real-time fly tracking system allowed for detailed analysis of the effects of these manipulations on behavior. For example, both hydrogen peroxide feeding and SOD over-expression increased all fly motion parameters, however, hydrogen peroxide feeding caused relatively more erratic movement, whereas SOD over-expression produced relatively faster-moving flies. Taken together, the data demonstrate that hydrogen peroxide has dramatic effects on fly movement and daily locomotor rhythms, and implicate hydrogen peroxide in the normal control of these processes.</p>
},
	keywords = {Animal, Animals, Behavior, Biological, Crosses, Drosophila melanogaster, Flight, Fluorescence, Genetic, Genetically Modified, Hydrogen Peroxide, Microscopy, Models, Movement, Oxidants, Oxidation-Reduction, Seasons, Superoxide Dismutase, Time Factors},
	issn = {1932-6203},
	doi = {10.1371/journal.pone.0007580},
	author = {Grover, Dhruv and Ford, Daniel and Brown, Christopher and Hoe, Nicholas and Erdem, Aysen and Tavar{\'e}, Simon and Tower, John}
}
