@article {65910,
	title = {An unbiased template of the Drosophila brain and ventral nerve cord.},
	journal = {PLoS One},
	volume = {15},
	year = {2020},
	month = {2020 Dec 31},
	pages = {e0236495},
	abstract = {<p>The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable analyzing many individuals in a common reference space. Several central brain templates exist for Drosophila, but every one is either biased, uses sub-optimal tissue preparation, is imaged at low resolution, or does not account for artifacts. No publicly available Drosophila ventral nerve cord template currently exists. In this work, we created high-resolution templates of the Drosophila brain and ventral nerve cord using the best-available technologies for imaging, artifact correction, stitching, and template construction using groupwise registration. We evaluated our central brain template against the four most competitive, publicly available brain templates and demonstrate that ours enables more accurate registration with fewer local deformations in shorter time.</p>
},
	issn = {1932-6203},
	doi = {10.1371/journal.pone.0236495},
	author = {Bogovic, John A and Otsuna, Hideo and Heinrich, Larissa and Ito, Masayoshi and Jeter, Jennifer and Meissner, Geoffrey and Nern, Aljoscha and Colonell, Jennifer and Malkesman, Oz and Ito, Kei and Saalfeld, Stephan}
}
@article {49386,
	title = {An unbiased template of the Drosophila brain and ventral nerve cord.},
	journal = {bioRxiv},
	year = {2018},
	month = {2018 Jul 25},
	abstract = {<p>The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individuals neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable analyzing many individuals in a common reference space. Several central brain templates exist for Drosophila, but every one is either biased, uses sub-optimal tissue preparation, is imaged at low resolution, or does not account for artifacts. No publicly available Drosophila ventral nerve cord template currently exists. In this work, we created high-resolution templates of the Drosophila brain and ventral nerve cord using the best-available technologies for imaging, artifact correction, stitching, and template construction using groupwise registration. We evaluated our central brain template against the four most competitive, publicly available brain templates and demonstrate that ours enables more accurate registration with fewer local deformations in shorter time.</p>
},
	doi = {10.1101/376384},
	author = {Bogovic, John A and Otsuna, Hideo and Heinrich, Larissa and Ito, Masayoshi and Jeter, Jennifer and Meissner, Geoffrey W and Nern, Aljoscha and Colonell, Jennifer and Malkesman, Oz and Saalfeld, Stephan}
}
