@article {45696, title = {A genetically specified connectomics approach applied to long-range feeding regulatory circuits.}, journal = {Nature Neuroscience}, volume = {17}, year = {2014}, month = {2014 Dec}, pages = {1830-9}, abstract = {

Synaptic connectivity and molecular composition provide a blueprint for information processing in neural circuits. Detailed structural analysis of neural circuits requires nanometer resolution, which can be obtained with serial-section electron microscopy. However, this technique remains challenging for reconstructing molecularly defined synapses. We used a genetically encoded synaptic marker for electron microscopy (GESEM) based on intra-vesicular generation of electron-dense labeling in axonal boutons. This approach allowed the identification of synapses from Cre recombinase-expressing or GAL4-expressing neurons in the mouse and fly with excellent preservation of ultrastructure. We applied this tool to visualize long-range connectivity of AGRP and POMC neurons in the mouse, two molecularly defined hypothalamic populations that are important for feeding behavior. Combining selective ultrastructural reconstruction of neuropil with functional and viral circuit mapping, we characterized some basic features of circuit organization for axon projections of these cell types. Our findings demonstrate that GESEM labeling enables long-range connectomics with molecularly defined cell types.

}, issn = {1546-1726}, doi = {10.1038/nn.3854}, author = {Atasoy, Deniz and Betley, J Nicholas and Li, Wei-Ping and Su, Helen H and Sertel, Sinem M and Scheffer, Louis K and Simpson, Julie H and Fetter, Richard D and Sternson, Scott M} } @article {5136, title = {Deconstruction of a neural circuit for hunger.}, journal = {Nature}, volume = {488}, year = {2012}, month = {2012 Aug 9}, pages = {172-7}, abstract = {

Hunger is a complex behavioural state that elicits intense food seeking and consumption. These behaviours are rapidly recapitulated by activation of starvation-sensitive AGRP neurons, which present an entry point for reverse-engineering neural circuits for hunger. Here we mapped synaptic interactions of AGRP neurons with multiple cell populations in mice and probed the contribution of these distinct circuits to feeding behaviour using optogenetic and pharmacogenetic techniques. An inhibitory circuit with paraventricular hypothalamus (PVH) neurons substantially accounted for acute AGRP neuron-evoked eating, whereas two other prominent circuits were insufficient. Within the PVH, we found that AGRP neurons target and inhibit oxytocin neurons, a small population that is selectively lost in Prader-Willi syndrome, a condition involving insatiable hunger. By developing strategies for evaluating molecularly defined circuits, we show that AGRP neuron suppression of oxytocin neurons is critical for evoked feeding. These experiments reveal a new neural circuit that regulates hunger state and pathways associated with overeating disorders.

}, issn = {1476-4687}, doi = {10.1038/nature11270}, author = {Atasoy, Deniz and Betley, J Nicholas and Su, Helen H and Sternson, Scott M} } @article {3977, title = {Chemical and genetic engineering of selective ion channel-ligand interactions.}, journal = {Science}, volume = {333}, year = {2011}, month = {2011 Sep 2}, pages = {1292-6}, abstract = {

Ionic flux mediates essential physiological and behavioral functions in defined cell populations. Cell type-specific activators of diverse ionic conductances are needed for probing these effects. We combined chemistry and protein engineering to enable the systematic creation of a toolbox of ligand-gated ion channels (LGICs) with orthogonal pharmacologic selectivity and divergent functional properties. The LGICs and their small-molecule effectors were able to activate a range of ionic conductances in genetically specified cell types. LGICs constructed for neuronal perturbation could be used to selectively manipulate neuron activity in mammalian brains in vivo. The diversity of ion channel tools accessible from this approach will be useful for examining the relationship between neuronal activity and animal behavior, as well as for cell biological and physiological applications requiring chemical control of ion conductance.

}, keywords = {5-HT3, Animals, Benzamides, Bicyclo Compounds, Brain, Feeding Behavior, Female, Glycine, HEK293 Cells, Humans, Inbred C57BL, Ion Channel Gating, Ligand-Gated Ion Channels, Ligands, Membrane Potentials, Mice, Mutagenesis, Neurons, Nicotinic, Patch-Clamp Techniques, Protein Binding, Protein Engineering, Protein Structure, Quinuclidines, Receptors, Recombinant Fusion Proteins, Serotonin, Small Molecule Libraries, Stereoisomerism, Tertiary}, issn = {1095-9203}, doi = {10.1126/science.1206606}, author = {Magnus, Christopher J and Lee, Peter H and Atasoy, Deniz and Su, Helen H and Looger, Loren L and Sternson, Scott M} } @article {3975, title = {Hunger states switch a flip-flop memory circuit via a synaptic AMPK-dependent positive feedback loop.}, journal = {Cell}, volume = {146}, year = {2011}, month = {2011 Sep 16}, pages = {992-1003}, abstract = {

Synaptic plasticity in response to changes in physiologic state is coordinated by hormonal signals across multiple neuronal cell types. Here, we combine cell-type-specific electrophysiological, pharmacological, and optogenetic techniques to dissect neural circuits and molecular pathways controlling synaptic plasticity onto AGRP neurons, a population that regulates feeding. We find that food deprivation elevates excitatory synaptic input, which is mediated by\ a presynaptic positive feedback loop involving AMP-activated protein kinase. Potentiation of glutamate release was triggered by the orexigenic hormone ghrelin and exhibited hysteresis, persisting for hours after ghrelin removal. Persistent activity was reversed by the anorexigenic hormone leptin, and optogenetic photostimulation demonstrated involvement of opioid release from POMC neurons. Based on these experiments, we propose a memory storage device for physiological state constructed from bistable synapses that are flipped between two sustained activity states by transient exposure to hormones signaling energy levels.

}, issn = {1097-4172}, doi = {10.1016/j.cell.2011.07.039}, author = {Yang, Yunlei and Atasoy, Deniz and Su, Helen H and Sternson, Scott M} }