@article {68758,
	title = {The Kinetochore-Microtubule Coupling Machinery Is Repurposed in Sensory Nervous System Morphogenesis},
	journal = {Developmental Cell},
	volume = {48},
	year = {2019},
	month = {03/2019},
	pages = {864-872.e7},
	abstract = {<p>Summary Dynamic coupling of microtubule ends to kinetochores, built on the centromeres of chromosomes, directs chromosome segregation during cell division. Here, we report that the evolutionarily ancient kinetochore-microtubule\&nbsp;coupling machine, the KMN (Knl1/Mis12/Ndc80-complex) network, plays a critical role in neuronal morphogenesis. We show that the KMN network concentrates in microtubule-rich dendrites of developing sensory neurons that collectively extend in a multicellular morphogenetic event that occurs during C.\&nbsp;elegans embryogenesis. Post-mitotic degradation of KMN components in sensory neurons disrupts dendritic extension, leading to patterning and functional defects in the sensory nervous system. Structure-guided mutations revealed that the molecular interface that couples kinetochores to spindle microtubules also functions in neuronal development. These results identify a cell-division-independent function for the chromosome-segregation machinery and define a microtubule-coupling-dependent event in sensory nervous system morphogenesis.</p>
},
	keywords = {Chromosome Segregation, dendrite, kinetochore, KMN network, Knl1, microtubule, Mis12 complex, Mitosis, Morphogenesis, Ndc80 complex, Nervous System, sensory neuron},
	issn = {1534-5807},
	doi = {https://doi.org/10.1016/j.devcel.2019.02.002},
	url = {https://www.sciencedirect.com/science/article/pii/S1534580719300577},
	author = {Dhanya K. Cheerambathur and Bram Prevo and Tiffany-Lynn Chow and Neil Hattersley and Shaohe Wang and Zhiling Zhao and Taekyung Kim and Adina Gerson-Gurwitz and Karen Oegema and Rebecca Green and Arshad Desai}
}
@article {68764,
	title = {A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions},
	journal = {Cell},
	volume = {165},
	year = {2016},
	month = {04/2016},
	pages = {396-409},
	abstract = {<p>Summary Multiple division cycles without growth are a characteristic feature of early embryogenesis. The female germline loads proteins and RNAs into oocytes to support these divisions, which lack many quality control mechanisms operating in somatic cells undergoing growth. Here, we describe a small RNA-Argonaute pathway that ensures early embryonic divisions in C.\&nbsp;elegans by employing catalytic slicing activity to broadly tune, instead of silence, germline gene expression. Misregulation of one target, a kinesin-13 microtubule depolymerase, underlies a major phenotype associated with pathway loss. Tuning of target transcript levels is guided by the density of homologous small RNAs, whose generation must ultimately be related to target sequence. Thus, the tuning action of a small RNA-catalytic Argonaute pathway generates oocytes capable of supporting embryogenesis. We speculate that the specialized nature of germline chromatin led to the emergence of small RNA-catalytic Argonaute pathways in the female germline as a post-transcriptional control layer to optimize oocyte composition.</p>
},
	keywords = {22G RNA, Argonaute, Centromere, chromosome, CSR-1, embryogenesis, Fertility, germline, maternal load, Oogenesis, small RNA},
	issn = {0092-8674},
	doi = {https://doi.org/10.1016/j.cell.2016.02.040},
	url = {https://www.sciencedirect.com/science/article/pii/S0092867416301908},
	author = {Adina Gerson-Gurwitz and Shaohe Wang and Shashank Sathe and Rebecca Green and Gene~W. Yeo and Karen Oegema and Arshad Desai}
}
