@article {68759,
	title = {CYK-4 functions independently of its centralspindlin partner ZEN-4 to cellularize oocytes in germline syncytia},
	journal = {eLife},
	volume = {7},
	year = {2018},
	month = {07/2018},
	pages = {e36919},
	abstract = {<p>Throughout metazoans, germ cells undergo incomplete cytokinesis to form syncytia connected by intercellular bridges. Gamete formation ultimately requires bridge closure, yet how bridges are reactivated to close is not known. The most conserved bridge component is centralspindlin, a complex of the Rho family GTPase-activating protein (GAP) CYK-4/MgcRacGAP and the microtubule motor ZEN-4/kinesin-6. Here, we show that oocyte production by the syncytial \textitCaenorhabditis elegans germline requires CYK-4 but not ZEN-4, which contrasts with cytokinesis, where both are essential. Longitudinal imaging after conditional inactivation revealed that CYK-4 activity is important for oocyte cellularization, but not for the cytokinesis-like events that generate syncytial compartments. CYK-4{\textquoteright}s lipid-binding C1 domain and the GTPase-binding interface of its GAP domain were both required to target CYK-4 to intercellular bridges and to cellularize oocytes. These results suggest that the conserved C1-GAP region of CYK-4 constitutes a targeting module required for closure of intercellular bridges in germline syncytia.</p>
},
	keywords = {centralspindlin, germline syncytia, intercellular bridge, Kinesin-6, MgcRacGAP, ring canal},
	issn = {2050-084X},
	doi = {10.7554/eLife.36919},
	url = {https://doi.org/10.7554/eLife.36919},
	author = {Lee, Kian-Yong and Green, Rebecca A and Gutierrez, Edgar and Gomez-Cavazos, J Sebastian and Kolotuev, Irina and Wang, Shaohe and Desai, Arshad and Groisman, Alex and Oegema, Karen},
	editor = {Balasubramanian, Mohan K and Akhmanova, Anna}
}
