@article {70414,
	title = {A workflow for the identification of oligomeric structures on tilted sample planes in Cryo-SMLM},
	journal = {bioRxiv},
	year = {2026},
	month = {2026 May 14},
	abstract = {<p>We introduce a workflow to identify oligomeric structures that are recorded with single-molecule localization microscopy (SMLM) under cryogenic conditions. Typically, these oligomers are assumed to consist of protomers arranged as equilateral two-dimensional polygons and every protomer is labeled with a dye molecule for visualization. Unlike previous work, we consider scenarios in which the sample plane has an unknown orientation relative to the focal plane. Our contribution is a high-precision plane-fitting algorithm to determine the sample plane, combined with geometrical transformations and two circle-fitting algorithms to identify the oligomeric structures. Our simulations on synthetic data demonstrate that the proposed workflow achieves high accuracy in estimating both the unknown tilted plane and the oligomer size.</p>
},
	doi = {10.64898/2026.05.12.724524},
	url = {https://www.biorxiv.org/content/early/2026/05/14/2026.05.12.724524},
	author = {Dong, Yakun and Yang, Zheyi and Schneider, Magdalena C. and Scherzer, Otmar and Sch{\"u}tz, Gerhard J.}
}
@article {68922,
	title = {Localization of fixed dipoles at high precision by accounting for sample drift during illumination},
	journal = {Applied Physics Letters},
	volume = {123},
	year = {2023},
	month = {2023 Jul 13},
	abstract = {<p>Single molecule localization microscopy relies on the precise quantification of the position of single dye emitters in a sample. This precision is improved by the number of photons that can be detected from each molecule. Particularly recording at cryogenic temperatures dramatically reduces photobleaching and would, hence, in principle, allow the user to massively increase the illumination time to several seconds. The downside of long illuminations, however, would be image blur due to inevitable jitter or drift occurring during the illuminations, which deteriorates the localization precision. In this paper, we theoretically demonstrate that a parallel recording of the fiducial marker beads together with a fitting approach accounting for the full drift trajectory allows for largely eliminating drift effects for drift magnitudes of several hundred nanometers per frame. We showcase the method for linear and diffusional drift as well as oscillations, assuming fixed dipole orientations during each illumination.</p>
},
	issn = {0003-6951},
	doi = {10.1063/5.0137834},
	url = {https://doi.org/10.1063/5.0137834},
	author = {Hinterer, Fabian and Schneider, Magdalena C. and Hubmer, Simon and L{\'o}pez-Mart{\'\i}nez, Montserrat and Ramlau, Ronny and Sch{\"u}tz, Gerhard J.}
}
