@article {69252,
	title = {Four-dimensional quantitative analysis of cell plate development using lattice light sheet microscopy identifies robust transition points between growth phases.},
	journal = {Journal of Experimental Botany},
	year = {2024},
	month = {2024 Mar 4},
	abstract = {<p>Cell plate formation during cytokinesis entails multiple stages occurring concurrently and requiring orchestrated vesicle delivery, membrane remodeling, and timely polysaccharide deposition, such as callose. Understanding such a dynamic process requires dissection in time and space; this has been a major hurdle in studying cytokinesis. Using lattice light sheet microscopy (LLSM) we studied cell plate development in four dimensions, through the behavior of the cytokinesis specific GTPase YFP-RABA2a vesicles. We monitored the entire length of cell plate development, from its first emergence, with the aid of YFP-RABA2a, both in the presence and absence of cytokinetic callose. By developing a robust cytokinetic vesicle volume analysis pipeline, we identified distinct behavioral patterns, allowing the identification of three easily trackable, cell plate developmental phases. Notably, the phase transition between phase I and phase II is striking, indicating a switch from membrane accumulation to the recycling of excess membrane material. We interrogated the role of callose using pharmacological inhibition with LLSM and electron microscopy. Loss of callose inhibited the phase transitions, establishing the critical role and timing of the polysaccharide deposition in cell plate expansion and maturation. This study exemplifies the power of combining LLSM with quantitative analysis to decode and untangle such a complex process.</p>
},
	issn = {1460-2431},
	doi = {10.1093/jxb/erae091},
	author = {Sinclair, Rosalie and Wang, Minmin and Jawaid, Muhammad Zaki and Longkumer, Toshisangba and Aaron, Jesse and Rossetti, Blair and Wait, Eric and McDonald, Kent and Cox, Daniel and Heddleston, John and Wilkop, Thomas and Drakakaki, Georgia}
}
@article {68959,
	title = {Dissecting Cell Plate Development During Plant Cytokinesis.},
	journal = {Microscopy and Microanalysis},
	volume = {29},
	year = {2023},
	month = {2023 Jul 22},
	pages = {865},
	keywords = {Cytokinesis},
	issn = {1435-8115},
	doi = {10.1093/micmic/ozad067.428},
	author = {Sinclair, Rosalie and Cox, Daniel and Heddleston, John and Aaron, Jesse and Wait, Eric and Wilkop, Thomas and Drakakaki, Georgia}
}
