@article {69217,
	title = {A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions.},
	journal = {bioRxiv},
	year = {2024},
	month = {2024 Feb 16},
	abstract = {<p>The endoplasmic reticulum (ER) is an important regulator of Ca2+\&nbsp;in cells and dysregulation of ER calcium homeostasis can lead to numerous pathologies. Understanding how various pharmacological and genetic perturbations of ER Ca2+\&nbsp;homeostasis impacts cellular physiology would likely be facilitated by more quantitative measurements of ER Ca2+\&nbsp;levels that allow easier comparisons across conditions. Here, we developed a ratiometric version of our original ER-GCaMP probe that allows for more quantitative comparisons of the concentration of Ca2+\&nbsp;in the ER across cell types and sub-cellular compartments. Using this approach we show that the resting concentration of ER Ca2+ in primary dissociated neurons is substantially lower than that in measured in embryonic fibroblasts.</p>
},
	doi = {10.1101/2024.02.15.580492},
	url = {https://doi.org/10.1101/2024.02.15.580492},
	author = {Ryan J. Farrell and Kirsten G. Bredvik and Michael B. Hoppa and S. Thomas Hennigan and Timothy A. Brown and Timothy A. Ryan}
}
