@article {4326,
	title = {Large-scale automated histology in the pursuit of connectomes.},
	journal = {The Journal of Neuroscience: The Official Journal of the Society for Neuroscience},
	volume = {31},
	year = {2011},
	month = {2011 Nov 9},
	pages = {16125-38},
	abstract = {<p>How does the brain compute? Answering this question necessitates neuronal connectomes, annotated graphs of all synaptic connections within defined brain areas. Further, understanding the energetics of the brain{\textquoteright}s computations requires vascular graphs. The assembly of a connectome requires sensitive hardware tools to measure neuronal and neurovascular features in all three dimensions, as well as software and machine learning for data analysis and visualization. We present the state of the art on the reconstruction of circuits and vasculature that link brain anatomy and function. Analysis at the scale of tens of nanometers yields connections between identified neurons, while analysis at the micrometer scale yields probabilistic rules of connection between neurons and exact vascular connectivity.</p>
},
	issn = {1529-2401},
	doi = {10.1523/JNEUROSCI.4077-11.2011},
	author = {Kleinfeld, David and Bharioke, Arjun and Blinder, Pablo and Bock, Davi D and Briggman, Kevin L and Chklovskii, Dmitri B and Denk, Winfried and Helmstaedter, Moritz and Kaufhold, John P and Lee, Wei-Chung Allen and Meyer, Hanno S and Micheva, Kristina D and Oberlaender, Marcel and Prohaska, Steffen and Reid, R Clay and Smith, Stephen J and Takemura, Shinya and Tsai, Philbert S and Sakmann, Bert}
}
@article {1342,
	title = {Whole-cell recordings in freely moving rats.},
	journal = {Neuron},
	volume = {51},
	year = {2006},
	month = {2006 Aug 17},
	pages = {399-407},
	abstract = {<p>Intracellular recording, which allows direct measurement of the membrane potential and currents of individual neurons, requires a very mechanically stable preparation and has thus been limited to in vitro and head-immobilized in vivo experiments. This restriction constitutes a major obstacle for linking cellular and synaptic physiology with animal behavior. To overcome this limitation we have developed a method for performing whole-cell recordings in freely moving rats. We constructed a miniature head-mountable recording device, with mechanical stabilization achieved by anchoring the recording pipette rigidly in place after the whole-cell configuration is established. We obtain long-duration recordings (mean of approximately 20 min, maximum 60 min) in freely moving animals that are remarkably insensitive to mechanical disturbances, then reconstruct the anatomy of the recorded cells. This head-anchored whole-cell recording technique will enable a wide range of new studies involving detailed measurement and manipulation of the physiological properties of identified cells during natural behaviors.</p>
},
	keywords = {Animal, Animals, Behavior, Brain, Electrodes, Implanted, Male, Membrane Potentials, Microelectrodes, Neurons, Newborn, Patch-Clamp Techniques, Rats, Sprague-Dawley, Wakefulness, Wistar},
	issn = {0896-6273},
	doi = {10.1016/j.neuron.2006.07.004},
	author = {Lee, Albert K and Manns, Ian D and Sakmann, Bert and Brecht, Michael}
}
