@article {69227,
	title = {A series of spontaneously blinking dyes for super-resolution microscopy},
	journal = {bioRxiv},
	year = {2024},
	month = {2024 Feb 24},
	abstract = {<p>Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.</p>
},
	doi = {10.1101/2024.02.23.581625},
	url = {https://www.biorxiv.org/content/early/2024/02/24/2024.02.23.581625},
	author = {Katie L. Holland and Sarah E. Plutkis and Timothy A. Daugird and Abhishek Sau and Jonathan B. Grimm and Brian P. English and Qinsi Zheng and Sandeep Dave and Fariha Rahman and Liangqi Xie and Peng Dong and Ariana N. Tkachuk and Timothy A. Brown and Robert H. Singer and Zhe Liu and Catherine G. Galbraith and Siegfried M. Musser and Wesley R. Legant and Luke D. Lavis}
}
@article {69421,
	title = {YAP condensates are highly organized hubs},
	journal = {iScience},
	year = {2024},
	month = {2024 May 07},
	pages = {109927},
	abstract = {<p>YAP/TEAD signaling is essential for organismal development, cell proliferation, and cancer progression. As a transcriptional coactivator, how YAP activates its downstream target genes is incompletely understood. YAP forms biomolecular condensates in response to hyperosmotic stress, concentrating transcription-related factors to activate downstream target genes. However, whether YAP forms condensates under other signals, how YAP condensates organize and function, and how YAP condensates activate transcription in general are unknown. Here, we report that endogenous YAP forms sub-micron scale condensates in response to Hippo pathway regulation and actin cytoskeletal tension. YAP condensates are stabilized by the transcription factor TEAD1, and recruit BRD4, a coactivator that is enriched at active enhancers. Using single-particle tracking, we found that YAP condensates slowed YAP diffusion within condensate boundaries, a possible mechanism for promoting YAP target search. These results reveal that YAP condensate formation is a highly regulated process that is critical for YAP/TEAD target gene expression.</p>
},
	issn = {2589-0042},
	doi = {https://doi.org/10.1016/j.isci.2024.109927},
	url = {https://www.sciencedirect.com/science/article/pii/S2589004224011490},
	author = {Siyuan Hao and Ye Jin Lee and Nadav Benhamou Goldfajn and Eduardo Flores and Jindayi Liang and Hannah Fuehrer and Justin Demmerle and Jennifer Lippincott-Schwartz and Zhe Liu and Shahar Sukenik and Danfeng Cai}
}
