@article {70429,
	title = {Abstract 5550: Phase diversity wavefront sensing for aberration correction in light sheet microscopy},
	journal = {Journal of Biological Chemistry},
	volume = {302},
	year = {2026},
	month = {2026 May },
	pages = {112990},
	abstract = {<p>Fluorescence microscopy provides insights into cellular structure and function, but the undesirable bending of light from the sample or imperfect optics ({\textquotedblleft}optical aberrations{\textquotedblright}) often degrade imaging resolution or signal. Adaptive Optics (AO) techniques that sense and subsequently correct the aberrations can restore diffraction-limited imaging, but most implementations remain technically complex and expensive. Wavefront sensing, the key step in AO, remains particularly challenging due to slow speed or additional illumination dose imparted to the sample. Previously we showed that phase diversity (PD), a method for wavefront sensing originally developed for use in astronomy, can rapidly sense and correct aberrations in a widefield fluorescence microscope. Here we extend this approach to light sheet microscopy, showing that the PD-based AO of sample-induced aberrations in live Caenorhabditis elegans embryos restores image contrast and resolution, enabling subcellular imaging throughout the imaging volume. In addition to these preliminary results, the further improvement and extension of this technology to additional samples and microscopes will be discussed.</p>
},
	keywords = {Adaptive optics, Fluorescence microscopy, light-sheet microscopy},
	issn = {0021-9258},
	doi = {https://doi.org/10.1016/j.jbc.2026.112990},
	url = {https://www.sciencedirect.com/science/article/pii/S0021925826018624},
	author = {Courtney Johnson and Magdalena Schneider and Ryan Christensen and Xuesong Li and Min Guo and Patrick La Riviere and Hari Shroff}
}
@article {69282,
	title = {Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM)},
	journal = {bioRxiv},
	year = {2024},
	month = {2024 Mar 12},
	abstract = {<p>Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the three-dimensional orientations and diffraction-limited positions of ensembles of fluorescent dipoles that label biological structures, and we share a set of visualization, histogram, and profiling tools for interpreting these positions and orientations. We model our samples, their excitation, and their detection using coarse-grained representations we call orientation distribution functions (ODFs). We apply ODFs to create physics-informed models of image formation with spatio-angular point-spread and transfer functions. We use theory and experiment to conclude that light-sheet tilting is a necessary part of our design for recovering all three-dimensional orientations. We use our system to extend known two-dimensional results to three dimensions in FM1-43-labelled giant unilamellar vesicles, fast-scarlet-labelled cellulose in xylem cells, and phalloidin-labelled actin in U2OS cells. Additionally, we observe phalloidin-labelled actin in mouse fibroblasts grown on grids of labelled nanowires and identify correlations between local actin alignment and global cell-scale orientation, indicating cellular coordination across length scales.Competing Interest StatementH.S., A.K., S.M., P.L.R., R.O., Y.W., and T.C. hold US Patent $\#$11428632.</p>
},
	doi = {10.1101/2024.03.09.584243},
	url = {https://www.biorxiv.org/content/early/2024/03/12/2024.03.09.584243},
	author = {Talon Chandler and Min Guo and Yijun Su and Jiji Chen and Yicong Wu and Junyu Liu and Atharva Agashe and Robert S. Fischer and Shalin B. Mehta and Abhishek Kumar and Tobias I. Baskin and Valentin Jamouille and Huafeng Liu and Vinay Swaminathan and Amrinder Nain and Rudolf Oldenbourg and Patrick La Riviere and Hari Shroff}
}
