@article {69345,
	title = {Differential uptake of MRI contrast agents indicates charge-selective blood-brain interface in the crayfish},
	journal = {Cell and Tissue Research},
	volume = {349},
	year = {2012},
	month = {2012 Apr 19},
	pages = {493 - 503},
	abstract = {<p>This study provides a new perspective on the long-standing problem of the nature of the decapod crustacean blood-brain interface. Previous studies of crustacean blood-brain interface permeability have relied on invasive histological, immunohistochemical and electrophysiological techniques, indicating a leaky non-selective blood-brain barrier. The present investigation involves the use of magnetic resonance imaging (MRI), a method for non-invasive longitudinal tracking of tracers in real-time. Differential uptake rates of two molecularly distinct MRI contrast agents, namely manganese (Mn(II)) and Magnevist{\textregistered} (Gd-DTPA), were observed and quantified in the crayfish,\&nbsp;<em>Cherax destructor</em>. Contrast agents were injected into the pericardium and uptake was observed with longitudinal MRI for approximately 14.5\&nbsp;h. Mn(II) was taken up quickly into neural tissue (within 6.5\&nbsp;min), whereas Gd-DTPA was not taken up into neural tissue and was instead restricted to the intracerebral vasculature or excreted into nearby sinuses. Our results provide evidence for a charge-selective intracerebral blood-brain interface in the crustacean nervous system, a structural characteristic once considered too complex for a lower-order arthropod.</p>
},
	issn = {0302-766X},
	doi = {10.1007/s00441-012-1413-9},
	url = {http://link.springer.com/10.1007/s00441-012-1413-9http://link.springer.com/content/pdf/10.1007/s00441-012-1413-9http://link.springer.com/content/pdf/10.1007/s00441-012-1413-9.pdfhttp://link.springer.com/article/10.1007/s00441-012-1413-9/fulltext.html},
	author = {Otopalik, Adriane G. and Shin, Jane and Beltz, Barbara S. and Sandeman, David C. and Kolodny, Nancy H.}
}
