@article {49470,
	title = {Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population imaging in vivo.},
	journal = {PloS One},
	volume = {13},
	year = {2018},
	month = {2018},
	pages = {e0205444},
	abstract = {<p>Calcium imaging is commonly used to measure the neural activity of large groups of neurons in mice. Genetically encoded calcium indicators (GECIs) can be delivered for this purpose using non-invasive genetic methods. Compared to viral gene transfer, transgenic targeting of GECIs provides stable long-term expression and obviates the need for invasive viral injections. Transgenic mice expressing the green GECI GCaMP6 are already widely used. Here we present the generation and characterization of transgenic mice expressing the sensitive red GECI jRGECO1a, driven by the Thy1 promoter. Four transgenic lines with different expression patterns showed sufficiently high expression for cellular in vivo imaging. We used two-photon microscopy to characterize visual responses of individual neurons in the visual cortex in vivo. The signal-to-noise ratio in transgenic mice was comparable to, or better than, mice transduced with adeno-associated virus. In addition, we show that Thy1-jRGECO1a transgenic mice are useful for transcranial population imaging and functional mapping using widefield fluorescence microscopy. We also demonstrate imaging of visual responses in retinal ganglion cells in vitro. Thy1-jRGECO1a transgenic mice are therefore a useful addition to the toolbox for imaging activity in intact neural networks.</p>
},
	issn = {1932-6203},
	doi = {10.1371/journal.pone.0205444},
	author = {Dana, Hod and Novak, Ondrej and Guardado-Montesino, Michael and Fransen, James W and Hu, Amy and Borghuis, Bart G and Guo, Caiying and Kim, Douglas S and Svoboda, Karel}
}
@article {65012,
	title = {T cells require Foxo1 to populate the peripheral lymphoid organs.},
	journal = {Eur J Immunol},
	volume = {39},
	year = {2009},
	month = {2009 Oct 30},
	pages = {2991-9},
	abstract = {<p>Forkhead transcription factors play critical roles in leukocyte homeostasis. To study further the immunological functions of Foxo1, we generated mice that selectively lack Foxo1 in T cells (Foxo1(flox/flox) Lck.cre(+)conditional knockout mice (cKO)). Although thymocyte development appeared relatively normal, Foxo1 cKO mice harbored significantly increased percentages of mature single positive T cells in the thymus as compared with WT mice, yet possessed smaller lymph nodes and spleens that contained fewer T cells. Foxo1 cKO T cells were not more prone to apoptosis, but instead were characterized by a CD62L(lo) CCR7(lo) CD44(hi) surface phenotype, a poorly populated lymphoid compartment in the periphery, and were relatively refractory to TCR stimulation, all of which were associated with reduced expression of Sell, Klf2, Ccr7, and S1pr1. Thus, Foxo1 is critical for na{\"\i}ve T cells to populate the peripheral lymphoid organs by coordinating a molecular program that maintains homeostasis and regulates trafficking.</p>
},
	keywords = {Animals, Apoptosis, Cell Proliferation, Chemotaxis, Leukocyte, Flow Cytometry, Forkhead Box Protein O1, Forkhead Transcription Factors, Lymphoid Tissue, Mice, Mice, Knockout, Phenotype, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocyte Subsets, T-Lymphocytes},
	issn = {1521-4141},
	doi = {10.1002/eji.200939427},
	author = {Gubbels Bupp, Melanie R and Edwards, Bonnie and Guo, Caiying and Wei, Datsen and Chen, Gang and Wong, Brian and Masteller, Emma and Peng, Stanford L}
}
