@article {67648,
	title = {Molecular characterization of projection neuron subtypes in the mouse olfactory bulb.},
	journal = {eLife},
	volume = {10},
	year = {2021},
	month = {2021 Jul 22},
	abstract = {<p>Projection neurons (PNs) in the mammalian olfactory bulb (OB) receive input from the nose and project to diverse cortical and subcortical areas. Morphological and physiological studies have highlighted functional heterogeneity, yet no molecular markers have been described that delineate PN subtypes. Here, we used viral injections into olfactory cortex and fluorescent nucleus sorting to enrich PNs for high-throughput single nucleus and bulk RNA deep sequencing. Transcriptome analysis and RNA hybridization identified distinct mitral and tufted cell populations with characteristic transcription factor network topology, cell adhesion and excitability-related gene expression. Finally, we describe a new computational approach for integrating bulk and snRNA-seq data, and provide evidence that different mitral cell populations preferentially project to different target regions. Together, we have identified potential molecular and gene regulatory mechanisms underlying PN diversity and provide new molecular entry points into studying the diverse functional roles of mitral and tufted cell subtypes.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.65445},
	author = {Zeppilli, Sara and Ackels, Tobias and Attey, Robin and Klimpert, Nell and Kimberly Ritola, Dr and Boeing, Stefan and Crombach, Anton and Schaefer, Andreas T and Fleischmann, Alexander}
}
@article {4305,
	title = {Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo.},
	journal = {Nature Neuroscience},
	volume = {14},
	year = {2011},
	month = {2011 Aug},
	pages = {1089-93},
	abstract = {<p>Multiphoton imaging (MPI) is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. We combined regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend MPI of neuronal population activity into layer 5 (L5) of adult mouse somatosensory cortex. We found that this approach could be used to record and quantify spontaneous and sensory-evoked activity in populations of L5 neuronal somata located as much as 800 μm below the pia. In addition, we found that RAMM could be used to simultaneously image activity from large (80) populations of apical dendrites and follow these dendrites down to their somata of origin.</p>
},
	keywords = {Animals, Axons, Calcium, Dendrites, Evoked Potentials, Green Fluorescent Proteins, Inbred C57BL, Mice, Nerve Net, Neurons, Newborn, Photons, Physical Stimulation, Probability, Reaction Time, Somatosensory, Somatosensory Cortex, Statistics as Topic, Transgenic},
	issn = {1546-1726},
	doi = {10.1038/nn.2879},
	author = {Mittmann, Wolfgang and Wallace, Damian J and Czubayko, Uwe and Herb, Jan T and Schaefer, Andreas T and Looger, Loren L and Denk, Winfried and Kerr, Jason N D}
}
