@article {2677,
	title = {Molecular mechanisms of corticosteroid synergy with thyroid hormone during tadpole metamorphosis.},
	journal = {General and Comparative Endocrinology},
	volume = {168},
	year = {2010},
	month = {2010 Sep 1},
	pages = {209-19},
	abstract = {<p>Corticosteroids (CS) act synergistically with thyroid hormone (TH) to accelerate amphibian metamorphosis. Earlier studies showed that CS increase nuclear 3,5,3{\textquoteright}-triiodothyronine (T(3)) binding capacity in tadpole tail, and 5{\textquoteright} deiodinase activity in tadpole tissues, increasing the generation of T(3) from thyroxine (T(4)). In the present study we investigated CS synergy with TH by analyzing expression of key genes involved in TH and CS signaling using tadpole tail explant cultures, prometamorphic tadpoles, and frog tissue culture cells (XTC-2 and XLT-15). Treatment of tail explants with T(3) at 100 nM, but not at 10 nM caused tail regression. Corticosterone (CORT) at three doses (100, 500 and 3400 nM) had no effect or increased tail size. T(3) at 10 nM plus CORT caused tails to regress similar to 100 nM T(3). Thyroid hormone receptor beta (TRbeta) mRNA was synergistically upregulated by T(3) plus CORT in tail explants, tail and brain in vivo, and tissue culture cells. The activating 5{\textquoteright} deiodinase type 2 (D2) mRNA was induced by T(3) and CORT in tail explants and tail in vivo. Thyroid hormone increased expression of glucocorticoid (GR) and mineralocorticoid receptor (MR) mRNAs. Our findings support that the synergistic actions of TH and CS in metamorphosis occur at the level of expression of genes for TRbeta and D2, enhancing tissue sensitivity to TH. Concurrently, TH enhances tissue sensitivity to CS by upregulating GR and MR. Environmental stressors can modulate the timing of tadpole metamorphosis in part by CS enhancing the response of tadpole tissues to the actions of TH.</p>
},
	keywords = {Adrenal Cortex Hormones, Animals, Biological, Cell Line, Drug Synergism, Larva, Metamorphosis, Reverse Transcriptase Polymerase Chain Reaction, Triiodothyronine, Xenopus},
	issn = {1095-6840},
	doi = {10.1016/j.ygcen.2010.03.014},
	author = {Bonett, Ronald M and Hoopfer, Eric D and Denver, Robert J}
}
