@article {65382, title = {Conservation and divergence of related neuronal lineages in the central brain.}, journal = {eLife}, volume = {9}, year = {2020}, month = {2020 Apr 07}, abstract = {

Wiring a complex brain requires many neurons with intricate cell specificity, generated by a limited number of neural stem cells. central brain lineages are a predetermined series of neurons, born in a specific order. To understand how lineage identity translates to neuron morphology, we mapped 18 central brain lineages. While we found large aggregate differences between lineages, we also discovered shared patterns of morphological diversification. Lineage identity plus Notch-mediated sister fate govern primary neuron trajectories, whereas temporal fate diversifies terminal elaborations. Further, morphological neuron types may arise repeatedly, interspersed with other types. Despite the complexity, related lineages produce similar neuron types in comparable temporal patterns. Different stem cells even yield two identical series of dopaminergic neuron types, but with unrelated sister neurons. Together, these phenomena suggest that straightforward rules drive incredible neuronal complexity, and that large changes in morphology can result from relatively simple fating mechanisms.

}, issn = {2050-084X}, doi = {10.7554/eLife.53518}, author = {Lee, Ying-Jou and Yang, Ching-Po and Miyares, Rosa L and Huang, Yu-Fen and He, Yisheng and Ren, Qingzhong and Chen, Hui-Min and Kawase, Takashi and Ito, Masayoshi and Otsuna, Hideo and Sugino, Ken and Aso, Yoshi and Ito, Kei and Lee, Tzumin} } @article {6876, title = {Drosophila intermediate neural progenitors produce lineage-dependent related series of diverse neurons.}, journal = {Development}, volume = {141}, year = {2014}, month = {2014 Jan}, pages = {253-8}, abstract = {

Drosophila type II neuroblasts (NBs), like mammalian neural stem cells, deposit neurons through intermediate neural progenitors (INPs) that can each produce a series of neurons. Both type II NBs and INPs exhibit age-dependent expression of various transcription factors, potentially specifying an array of diverse neurons by combinatorial temporal patterning. Not knowing which mature neurons are made by specific INPs, however, conceals the actual variety of neuron types and limits further molecular studies. Here we mapped neurons derived from specific type II NB lineages and found that sibling INPs produced a morphologically similar but temporally regulated series of distinct neuron types. This suggests a common fate diversification program operating within each INP that is modulated by NB age to generate slightly different sets of diverse neurons based on the INP birth order. Analogous mechanisms might underlie the expansion of neuron diversity via INPs in mammalian brain.

}, keywords = {Animals, Brain, Cell Lineage, Drosophila, Genetically Modified, Models, Neural Stem Cells, Neurogenesis, Neurological}, issn = {1477-9129}, doi = {10.1242/dev.103069}, author = {Wang, Yu-Chun and Yang, Jacob S and Johnston, Rebecca and Ren, Qingzhong and Lee, Ying-Jou and Luan, Haojiang and Brody, Thomas and Odenwald, Ward F and Lee, Tzumin} } @article {6898, title = {Making Drosophila lineage-restricted drivers via patterned recombination in neuroblasts.}, journal = {Nature Neuroscience}, volume = {17}, year = {2014}, month = {2014 Apr}, pages = {631-7}, abstract = {

The Drosophila cerebrum originates from about 100 neuroblasts per hemisphere, with each neuroblast producing a characteristic set of neurons. Neurons from a neuroblast are often so diverse that many neuron types remain unexplored. We developed new genetic tools that target neuroblasts and their diverse descendants, increasing our ability to study fly brain structure and development. Common enhancer-based drivers label neurons on the basis of terminal identities rather than origins, which provides limited labeling in the heterogeneous neuronal lineages. We successfully converted conventional drivers that are temporarily expressed in neuroblasts, into drivers expressed in all subsequent neuroblast progeny. One technique involves immortalizing GAL4 expression in neuroblasts and their descendants. Another depends on loss of the GAL4 repressor, GAL80, from neuroblasts during early neurogenesis. Furthermore, we expanded the diversity of MARCM-based reagents and established another site-specific mitotic recombination system. Our transgenic tools can be combined to map individual neurons in specific lineages of various genotypes.

}, issn = {1546-1726}, doi = {10.1038/nn.3654}, author = {Awasaki, Takeshi and Kao, Chih-Fei and Lee, Ying-Jou and Yang, Ching-Po and Huang, Yaling and Pfeiffer, Barret D and Luan, Haojiang and Jing, Xiaotang and Huang, Yu-Fen and He, Yisheng and Schroeder, Mark David and Kuzin, Alexander and Brody, Thomas and Zugates, Christopher T and Odenwald, Ward F and Lee, Tzumin} }