@article {6360,
	title = {Knockdown of parhyale ultrabithorax recapitulates evolutionary changes in crustacean appendage morphology.},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {106},
	year = {2009},
	month = {2009 Aug 18},
	pages = {13892-6},
	abstract = {<p>Crustaceans possess remarkably diverse appendages, both between segments of a single individual as well as between species. Previous studies in a wide range of crustaceans have demonstrated a correlation between the anterior expression boundary of the homeotic (Hox) gene Ultrabithorax (Ubx) and the location and number of specialized thoracic feeding appendages, called maxillipeds. Given that Hox genes regulate regional identity in organisms as diverse as mice and flies, these observations in crustaceans led to the hypothesis that Ubx expression regulates the number of maxillipeds and that evolutionary changes in Ubx expression have generated various aspects of crustacean appendage diversity. Specifically, evolutionary changes in the expression boundary of Ubx have resulted in crustacean species with either 0, 1, 2, or 3 pairs of thoracic maxillipeds. Here we test this hypothesis by altering the expression of Ubx in Parhyale hawaiensis, a crustacean that normally possesses a single pair of maxillipeds. By reducing Ubx expression, we can generate Parhyale with additional maxillipeds in a pattern reminiscent of that seen in other crustacean species, and these morphological alterations are maintained as the animals molt and mature. These results provide critical evidence supporting the proposition that changes in Ubx expression have played a role in generating crustacean appendage diversity and lend general insights into the mechanisms of morphological evolution.</p>
},
	keywords = {Animals, Base Sequence, Biological, Biological Evolution, Cloning, Crustacea, Electron, Extremities, Gene Expression Regulation, Genes, Genetic Techniques, Homeobox, Homeodomain Proteins, In Situ Hybridization, Microscopy, Models, Molecular, Molecular Sequence Data, RNA, Scanning, Small Interfering},
	issn = {1091-6490},
	doi = {10.1073/pnas.0903105106},
	author = {Liubicich, Danielle M and Serano, Julia M and Pavlopoulos, Anastasios and Kontarakis, Zacharias and Protas, Meredith E and Kwan, Elaine and Chatterjee, Sandip and Tran, Khoa D and Averof, Michalis and Patel, Nipam H}
}
@article {6359,
	title = {Probing the evolution of appendage specialization by Hox gene misexpression in an emerging model crustacean.},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {106},
	year = {2009},
	month = {2009 Aug 18},
	pages = {13897-902},
	abstract = {<p>Changes in the expression of Hox genes have been widely linked to the evolution of animal body plans, but functional demonstrations of this relationship have been impeded by the lack of suitable model organisms. A classic case study involves the repeated evolution of specialized feeding appendages, called maxillipeds, from anterior thoracic legs, in many crustacean lineages. These leg-to-maxilliped transformations correlate with the loss of Ultrabithorax (Ubx) expression from corresponding segments, which is proposed to be the underlying genetic cause. To functionally test this hypothesis, we establish tools for conditional misexpression and use these to misexpress Ubx in the crustacean Parhyale hawaiensis. Ectopic Ubx leads to homeotic transformations of anterior appendages toward more posterior thoracic fates, including maxilliped-to-leg transformations, confirming the capacity of Ubx to control thoracic (leg) versus gnathal (feeding) segmental identities. We find that maxillipeds not only are specified in the absence of Ubx, but also can develop in the presence of low/transient Ubx expression. Our findings suggest a path for the gradual evolutionary transition from thoracic legs to maxillipeds, in which stepwise changes in Hox gene expression have brought about this striking morphological and functional transformation.</p>
},
	keywords = {Animals, Cloning, Crustacea, Down-Regulation, Electron, Extremities, Gene Expression Regulation, Genes, Genetic, Genetically Modified, Heat-Shock Proteins, Homeobox, Homeodomain Proteins, Immunohistochemistry, Microscopy, Models, Molecular, Phenotype, Scanning, Transgenes},
	issn = {1091-6490},
	doi = {10.1073/pnas.0902804106},
	author = {Pavlopoulos, Anastasios and Kontarakis, Zacharias and Liubicich, Danielle M and Serano, Julia M and Akam, Michael and Patel, Nipam H and Averof, Michalis}
}
