@article {68812,
	title = {A viral toolbox for conditional and transneuronal gene expression in zebrafish},
	journal = {eLife},
	volume = {11},
	year = {2022},
	month = {07/2022},
	pages = {e77153},
	abstract = {<p>The zebrafish is an important model in systems neuroscience but viral tools to dissect the structure and function of neuronal circuitry are not established. We developed methods for efficient gene transfer and retrograde tracing in adult and larval zebrafish by herpes simplex viruses (HSV1). HSV1 was combined with the Gal4/UAS system to target cell types with high spatial, temporal, and molecular specificity. We also established methods for efficient transneuronal tracing by modified rabies viruses in zebrafish. We demonstrate that HSV1 and rabies viruses can be used to visualize and manipulate genetically or anatomically identified neurons within and across different brain areas of adult and larval zebrafish. An expandable library of viruses is provided to express fluorescent proteins, calcium indicators, optogenetic probes, toxins and other molecular tools. This toolbox creates new opportunities to interrogate neuronal circuits in zebrafish through combinations of genetic and viral approaches.</p>
},
	keywords = {Gene Transfer, Imaging, neuronal circuit, viral vector},
	issn = {2050-084X},
	doi = {10.7554/eLife.77153},
	url = {https://doi.org/10.7554/eLife.77153},
	author = {Satou, Chie and Neve, Rachael L and Oyibo, Hassana K and Zmarz, Pawel and Huang, Kuo-Hua and Arn Bouldoires, Estelle and Mori, Takuma and Higashijima, Shin-Ichi and Keller, Georg B and Friedrich, Rainer W},
	editor = {Bagnall, Martha W and Stainier, Didier YR and Jin, Lei}
}
@article {2816,
	title = {Reconstruction of complementary images in second harmonic generation microscopy.},
	journal = {Optics Express},
	volume = {14},
	year = {2006},
	month = {2006 May 29},
	pages = {4727-35},
	abstract = {<p>Second harmonic generation microscopy(SHGM) has become widely used to image biological samples. Due to the complexity of biological samples, more and more effort has been put on polarization imaging in SHGM technology to uncover their structures. In this work, we put forward a novel stitching method based on careful mathematical calculation, and accomplish it by rotating laser polarization. We first show its validity in imaging a perfectly synthesized bio-origin polymer poly (3-hyroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Then, we test its power by getting a true image of fibrillar collagen structure of rat-tail tendon.</p>
},
	issn = {1094-4087},
	doi = {10.1364/AO.50.001792},
	author = {Gao, Liang and Jin, Lei and Xue, Ping and Xu, Jun and Wang, Yi and Ma, Hui and Chen, Dieyan}
}
