@article {65175,
	title = {Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis.},
	journal = {Development},
	volume = {146},
	year = {2019},
	month = {2019 Sep 30},
	abstract = {<p>Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with α-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms.</p>
},
	issn = {1477-9129},
	doi = {10.1242/dev.179127},
	author = {Sato, Yuko and Hilbert, Lennart and Oda, Haruka and Wan, Yinan and Heddleston, John M and Chew, Teng-Leong and Zaburdaev, Vasily and Keller, Philipp and Lionnet, Timoth{\'e}e and Vastenhouw, Nadine and Kimura, Hiroshi}
}
@article {1692,
	title = {Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system.},
	journal = {The Journal of Cell Biology},
	volume = {171},
	year = {2005},
	month = {2005 Nov 21},
	pages = {627-40},
	abstract = {<p>Spindle pole bodies (SPBs) provide a structural basis for genome inheritance and spore formation during meiosis in yeast. Upon carbon source limitation during sporulation, the number of haploid spores formed per cell is reduced. We show that precise spore number control (SNC) fulfills two functions. SNC maximizes the production of spores (1-4) that are formed by a single cell. This is regulated by the concentration of three structural meiotic SPB components, which is dependent on available amounts of carbon source. Using experiments and computer simulation, we show that the molecular mechanism relies on a self-organizing system, which is able to generate particular patterns (different numbers of spores) in dependency on one single stimulus (gradually increasing amounts of SPB constituents). We also show that SNC enhances intratetrad mating, whereby maximal amounts of germinated spores are able to return to a diploid lifestyle without intermediary mitotic division. This is beneficial for the immediate fitness of the population of postmeiotic cells.</p>
},
	keywords = {Biological, Carbon, Centromere, Computer Simulation, Cytoplasm, Dominant, Electron, Fungal, Gene Expression Regulation, Genes, Genetic, Genetic Linkage, Genotype, Green Fluorescent Proteins, Haploidy, Meiosis, Microscopy, Mitotic Spindle Apparatus, Models, Mutation, Open Reading Frames, Plasmids, Recombination, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Software, Spores, Time Factors, Video},
	issn = {0021-9525},
	doi = {10.1083/jcb.200507168},
	author = {Taxis, Christof and Keller, Philipp and Kavagiou, Zaharoula and Jensen, Lars Juhl and Colombelli, Julien and Bork, Peer and Stelzer, Ernst H K and Knop, Michael}
}
