@article {960,
	title = {Yan functions as a general inhibitor of differentiation and is negatively regulated by activation of the Ras1/MAPK pathway.},
	journal = {Cell},
	volume = {81},
	year = {1995},
	month = {1995 Jun 16},
	pages = {857-66},
	abstract = {<p>Drosophila yan has been postulated to act as an antagonist of the proneural signal mediated by the sevenless/Ras1/MAPK pathway. We have mutagenized the eight MAPK phosphorylation consensus sites of yan and examined the effects of overexpressing the mutant protein in transgenic flies and transfected S2 cultured cells. Our results suggest that phosphorylation by MAPK affects the stability and subcellular localization of yan, resulting in rapid down-regulation of yan activity. Furthermore, MAPK-mediated down-regulation of yan function appears to be critical for the proper differentiation of both neuronal and nonneuronal tissues throughout development, suggesting that yan is an essential component of a general timing mechanism controlling the competence of a cell to respond to inductive signals.</p>
},
	keywords = {Alleles, Animals, Binding Sites, Calcium-Calmodulin-Dependent Protein Kinases, Developmental, DNA-Binding Proteins, Down-Regulation, Drosophila, Drosophila Proteins, Eye, Eye Proteins, Gene Expression Regulation, GTP-Binding Proteins, Membrane Glycoproteins, Mutagenesis, Neurons, Phosphorylation, Receptor Protein-Tyrosine Kinases, Repressor Proteins, Signal Transduction, Site-Directed},
	issn = {0092-8674},
	doi = {10.1186/gb-2007-8-7-r145},
	author = {Rebay, I and Rubin, G M}
}
@article {963,
	title = {The activities of two Ets-related transcription factors required for Drosophila eye development are modulated by the Ras/MAPK pathway.},
	journal = {Cell},
	volume = {78},
	year = {1994},
	month = {1994 Jul 15},
	pages = {137-47},
	abstract = {<p>We show that the activities of two Ets-related transcription factors required for normal eye development in Drosophila, pointed and yan, are regulated by the Ras1/MAPK pathway. The pointed gene codes for two related proteins, and we show that one form is a constitutive activator of transcription, while the activity of the other form is stimulated by the Ras1/MAPK pathway. Mutation of the single consensus MAPK phosphorylation site in the second form abrogates this responsiveness. yan is a negative regulator of photoreceptor determination, and genetic data suggest that it acts as an antagonist of Ras1. We demonstrate that yan can repress transcription and that this repression activity is negatively regulated by the Ras1/MAPK signal, most likely through direct phosphorylation of yan by MAPK.</p>
},
	keywords = {Animals, Biological, Calcium-Calmodulin-Dependent Protein Kinases, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Extracellular Signal-Regulated MAP Kinases, Eye, Eye Proteins, Gene Expression Regulation, Genes, Genetic, Insect, Invertebrate, Models, Morphogenesis, Nerve Tissue Proteins, Phenotype, Photoreceptor Cells, Proto-Oncogene Proteins, Proto-Oncogene Proteins p21(ras), ras, Repressor Proteins, Signal Transduction, Transcription, Transcription Factors},
	issn = {0092-8674},
	doi = {10.1186/gb-2007-8-7-r145},
	author = {O{\textquoteright}Neill, E M and Rebay, I and Tjian, R and Rubin, G M}
}
