@article {65371,
	title = {CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila.},
	journal = {Nucleic Acids Research},
	year = {2020},
	month = {2020 Mar 18},
	abstract = {<p>The genome is the blueprint for an organism. Interrogating the genome, especially locating critical cis-regulatory elements, requires deletion analysis. This is conventionally performed using synthetic constructs, making it cumbersome and non-physiological. Thus, we created Cas9-mediated Arrayed Mutagenesis of Individual Offspring (CAMIO) to achieve comprehensive analysis of a targeted region of native DNA. CAMIO utilizes CRISPR that is spatially restricted to generate independent deletions in the intact Drosophila genome. Controlled by recombination, a single guide RNA is stochastically chosen from a set targeting a specific DNA region. Combining two sets increases variability, leading to either indels at 1-2 target sites or inter-target deletions. Cas9 restriction to male germ cells elicits autonomous double-strand-break repair, consequently creating offspring with diverse mutations. Thus, from a single population cross, we can obtain a deletion matrix covering a large expanse of DNA at both coarse and fine resolution. We demonstrate the ease and power of CAMIO by mapping 5{\textquoteright}UTR sequences crucial for chinmo{\textquoteright}s post-transcriptional regulation.</p>
},
	issn = {1362-4962},
	doi = {10.1093/nar/gkaa177},
	author = {Chen, Hui-Min and Marques, Jorge Garcia and Sugino, Ken and Wei, Dingjun and Miyares, Rosa Linda and Lee, Tzumin}
}
@article {65382,
	title = {Conservation and divergence of related neuronal lineages in the  central brain.},
	journal = {eLife},
	volume = {9},
	year = {2020},
	month = {2020 Apr 07},
	abstract = {<p>Wiring a complex brain requires many neurons with intricate cell specificity, generated by a limited number of neural stem cells. central brain lineages are a predetermined series of neurons, born in a specific order. To understand how lineage identity translates to neuron morphology, we mapped 18 central brain lineages. While we found large aggregate differences between lineages, we also discovered shared patterns of morphological diversification. Lineage identity plus Notch-mediated sister fate govern primary neuron trajectories, whereas temporal fate diversifies terminal elaborations. Further, morphological neuron types may arise repeatedly, interspersed with other types. Despite the complexity, related lineages produce similar neuron types in comparable temporal patterns. Different stem cells even yield two identical series of dopaminergic neuron types, but with unrelated sister neurons. Together, these phenomena suggest that straightforward rules drive incredible neuronal complexity, and that large changes in morphology can result from relatively simple fating mechanisms.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.53518},
	author = {Lee, Ying-Jou and Yang, Ching-Po and Miyares, Rosa L and Huang, Yu-Fen and He, Yisheng and Ren, Qingzhong and Chen, Hui-Min and Kawase, Takashi and Ito, Masayoshi and Otsuna, Hideo and Sugino, Ken and Aso, Yoshi and Ito, Kei and Lee, Tzumin}
}
