@article {69251,
	title = {The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain.},
	journal = {Cell Reports},
	volume = {43},
	year = {2024},
	month = {2024 Feb 29},
	pages = {113842},
	abstract = {<p>Understanding the cell-type composition and spatial organization of brain regions is crucial for interpreting brain computation and function. In the thalamus, the anterior thalamic nuclei (ATN) are involved in a wide variety of functions, yet the cell-type composition of the ATN remains unmapped at a single-cell and spatial resolution. Combining single-cell RNA sequencing, spatial transcriptomics, and multiplexed fluorescent in situ hybridization, we identify three discrete excitatory cell-type clusters that correspond to the known nuclei of the ATN and uncover marker genes, molecular pathways, and putative functions of these cell types. We further illustrate graded spatial variation along the dorsomedial-ventrolateral axis for all individual nuclei of the ATN and additionally demonstrate that the anteroventral nucleus exhibits spatially covarying protein products and long-range inputs. Collectively, our study reveals discrete and continuous cell-type organizational principles of the ATN, which will help to guide and interpret experiments on ATN computation and function.</p>
},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2024.113842},
	author = {Kapustina, Margarita and Zhang, Angela A and Tsai, Jennifer Y J and Bristow, Brianna N and Kraus, Larissa and Sullivan, Kaitlin E and Erwin, Sarah R and Wang, Lihua and Stach, Tara R and Clements, Jody and Lemire, Andrew L and Cembrowski, Mark S}
}
@article {49118,
	title = {Continuous variation within cell types of the nervous system.},
	journal = {Trends in Neurosciences},
	volume = {41},
	year = {2018},
	month = {2018 Mar 22},
	pages = {337-48},
	abstract = {<p>The brain is an organ of immense complexity. Next-generation RNA sequencing (RNA-seq) is becoming increasingly popular in the deconstruction of this complexity into distinct classes of {\textquoteright}cell types{\textquoteright}. Notably, in addition to revealing the organization of this distinct cell-type landscape, the technology has also begun to illustrate that continuous variation can be found within narrowly defined cell types. Here we summarize the evidence for graded transcriptomic heterogeneity being present, widespread, and functionally relevant in the nervous system. We explain how these graded differences can map onto higher-order organizational features and how they may reframe existing interpretations of higher-order heterogeneity. Ultimately, a multimodal approach incorporating continuously variable cell types will facilitate an accurate reductionist interpretation of the nervous system.</p>
},
	issn = {1878-108X},
	doi = {10.1016/j.tins.2018.02.010},
	author = {Cembrowski, Mark S and Menon, Vilas}
}
