@article {48730, title = {Volume imaging.}, volume = {B2}, number = {14/452,181}, year = {2017}, month = {2017 Aug 01}, edition = {G01J 4/00 (20060101); G02B 21/00 (20060101); G02B 21/36 (20060101)}, abstract = {

A system for a laser-scanning microscope includes an optical element configured to transmit light in a first direction onto a first beam path and to reflect light in a second direction to a second beam path that is different from the first beam path; a reflector on the first beam path; and a lens including a variable focal length, the lens positioned on the first beam path. The lens and reflector are positioned relative to each other to cause light transmitted by the optical element to pass through the lens a plurality of times and in a different direction each time. In some implementations, the system also can include a feedback system that receives a signal that represents an amount of focusing of the lens, and changes the focal length of the lens based on the received signal.

}, url = {http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2\&Sect2=HITOFF\&u=\%2Fnetahtml\%2FPTO\%2Fsearch-adv.htm\&r=1\&p=1\&f=G\&l=50\&d=PTXT\&S1=(\%22Howard+Hughes+medical\%22.ASNM.)\&OS=AN/\%22Howard+Hughes+medical\%22\&RS=AN/\%22Howard+Hughes+medical\%22}, author = {Cui, Meng and Kong, Lingjie} } @article {47973, title = {Motion quantification during multi-photon functional imaging in behaving animals.}, journal = {Biomedical Optics Express}, volume = {7}, year = {2016}, month = {2016 Sep 1}, pages = {3686-3695}, abstract = {

Functional imaging in behaving animals is essential to understanding brain function. However, artifacts resulting from animal motion, including locomotion, can severely corrupt functional measurements. To dampen tissue motion, we designed a new optical window with minimal optical aberrations. Using the newly developed high-speed continuous volumetric imaging system based on an optical phase-locked ultrasound lens, we quantified motion of the cerebral cortex and hippocampal surface during two-photon functional imaging in behaving mice. We find that the out-of-plane motion is generally greater than the axial dimension of the point-spread-function during mouse locomotion, which indicates that high-speed continuous volumetric imaging is necessary to minimize motion artifacts.

}, doi = {10.1364/BOE.7.003686}, author = {Kong, Lingjie and Little, Justin P and Cui, Meng} } @article {46890, title = {Continuous volumetric imaging via an optical phase-locked ultrasound lens.}, journal = {Nature Methods}, volume = {12}, year = {2015}, month = {2015-Jul 13}, pages = {759-62}, abstract = {

In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficking of immune cells.

}, doi = {10.1038/nmeth.3476}, author = {Kong, Lingjie and Tang, Jianyong and Little, Justin P and Yu, Yang and L{\"a}mmermann, Tim and Lin, Charles P and Germain, Ronald N and Cui, Meng} } @article {46895, title = {A high throughput (>90\%), large compensation range, single-prism femtosecond pulse compressor.}, journal = {arXiv}, year = {2015}, month = {2015 Jun 20}, pages = {arXiv:1306.5011 }, abstract = {

We demonstrate a high throughput, large compensation range, single-prism femtosecond pulse compressor, using a single prism and two roof mirrors. The compressor has zero angular dispersion, zero spatial dispersion, zero pulse-front tilt, and unity magnification. The high efficiency is achieved by adopting two roof mirrors as the retroreflectors. We experimentally achieved ~ -14500 fs2 group delay dispersion (GDD) with 30 cm of prism tip-roof mirror prism separation, and ~90.7\% system throughput with the current implementation. With better components, the throughput can be even higher.

}, url = {http://arxiv.org/abs/1306.5011}, author = {Kong, Lingjie and Cui, Meng} } @article {46534, title = {In vivo neuroimaging through the highly scattering tissue via iterative multi-photon adaptive compensation technique.}, journal = {Optics Express}, volume = {23}, year = {2015}, month = {2015 Mar 9}, pages = {6145-50}, abstract = {

For in vivo deep tissue imaging, high order wavefront measurement and correction is needed for handling the severe wavefront distortion. Towards such a goal, we have developed the iterative multi-photon adaptive compensation technique (IMPACT). In this work, we explore using IMPACT to perform calcium imaging of neocortex through the intact skull of adult mice, and to image through the highly scattering white matter on the hippocampus surface.

}, issn = {1094-4087}, doi = {10.1364/OE.23.006145}, author = {Kong, Lingjie and Cui, Meng} } @article {45786, title = {In vivo fluorescence microscopy via iterative multi-photon adaptive compensation technique.}, journal = {Optical Express}, volume = {22}, year = {2014}, month = {2014 Oct 6}, pages = {23786-94}, abstract = {

Iterative multi-photon adaptive compensation technique (IMPACT) has been developed for wavefront measurement and compensation in highly scattering tissues. Our previous report was largely based on the measurements of fixed tissue. Here we demonstrate the advantages of IMPACT for in vivo imaging and report the latest results. In particular, we show that IMPACT can be used for functional imaging of awake mice, and greatly improve the in vivo neuron imaging in mouse cortex at large depth (~660 microns). Moreover, IMPACT enables neuron imaging through the intact skull of adult mice, which promises noninvasive optical measurements in mouse brain.

}, issn = {1094-4087}, doi = {10.1364/OE.22.023786}, author = {Kong, Lingjie and Cui, Meng} }