@article {65599,
	title = {Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy.},
	journal = {Neuron},
	volume = {106},
	year = {2020},
	month = {2020 May 06},
	pages = {369-387},
	abstract = {<p>Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.</p>
},
	issn = {1097-4199},
	doi = {10.1016/j.neuron.2020.03.004},
	author = {Ueda, Hiroki R and Dodt, Hans-Ulrich and Osten, Pavel and Economo, Michael N and Chandrashekar, Jayaram and Keller, Philipp J}
}
@article {65633,
	title = {Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy.},
	journal = {Neuron},
	volume = {106},
	year = {2020},
	month = {2020 May 06},
	pages = {369-387},
	abstract = {<p>Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.</p>
},
	issn = {1097-4199},
	doi = {10.1016/j.neuron.2020.03.004},
	author = {Ueda, Hiroki R and Dodt, Hans-Ulrich and Osten, Pavel and Economo, Michael N and Chandrashekar, Jayaram and Keller, Philipp J}
}
