@article {67812,
	title = {Towards community-driven metadata standards for light microscopy: tiered specifications extending the OME model.},
	journal = {Nature Methods},
	volume = {18},
	year = {2021},
	month = {2021 Dec 03},
	pages = {1427-1440},
	issn = {1548-7105},
	doi = {10.1038/s41592-021-01327-9},
	author = {Hammer, Mathias and Huisman, Maximiliaan and Rigano, Alessandro and Boehm, Ulrike and Chambers, James J and Gaudreault, Nathalie and North, Alison J and Pimentel, Jaime A and Sudar, Damir and Bajcsy, Peter and Brown, Claire M and Corbett, Alexander D and Faklaris, Orestis and Lacoste, Judith and Laude, Alex and Nelson, Glyn and Nitschke, Roland and Farzam, Farzin and Smith, Carlas S and Grunwald, David and Strambio-De-Castillia, Caterina}
}
@article {49435,
	title = {Integration of parallel opposing memories underlies memory extinction.},
	journal = {Cell},
	volume = {175},
	year = {2018},
	month = {2018 Sep 06},
	pages = {709-22},
	abstract = {<p>Accurately predicting an outcome requires that animals learn supporting and conflicting evidence from sequential experience. In mammals and invertebrates, learned fear responses can be suppressed by experiencing predictive cues without punishment,\&nbsp;a process called memory extinction. Here, we show that extinction of aversive memories in Drosophila requires specific dopaminergic neurons, which indicate that omission of punishment is remembered as a positive experience. Functional imaging revealed co-existence of intracellular calcium traces in different places in the mushroom body output neuron network for both the original aversive memory and a new appetitive extinction memory. Light and ultrastructural anatomy are consistent with parallel competing memories being combined within mushroom body output neurons that direct avoidance. Indeed, extinction-evoked plasticity in a pair of these neurons neutralizes the potentiated odor response imposed in the network by aversive learning. Therefore, flies track the accuracy of learned expectations by accumulating and integrating memories of conflicting events.</p>
},
	issn = {1097-4172},
	doi = {10.1016/j.cell.2018.08.021},
	author = {Felsenberg, Johannes and Jacob, Pedro F and Walker, Thomas and Barnstedt, Oliver and Edmondson-Stait, Amelia J and Pleijzier, Markus W and Otto, Nils and Schlegel, Philipp and Sharifi, Nadiya and Perisse, Emmanuel and Smith, Carlas S and Lauritzen, J Scott and Costa, Marta and Jefferis, Gregory S X E and Bock, Davi D and Waddell, Scott}
}
@article {46925,
	title = {Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking.},
	journal = {Journal of Cell Biology},
	volume = {209},
	year = {2015},
	month = {2015 May 25},
	pages = {609-19},
	abstract = {<p>Imaging single proteins or RNAs allows direct visualization of the inner workings of the cell. Typically, three-dimensional (3D) images are acquired by sequentially capturing a series of 2D sections. The time required to step through the sample often impedes imaging of large numbers of rapidly moving molecules. Here we applied multifocus microscopy (MFM) to instantaneously capture 3D single-molecule real-time images in live cells, visualizing cell nuclei at 10 volumes per second. We developed image analysis techniques to analyze messenger RNA (mRNA) diffusion in the entire volume of the nucleus. Combining MFM with precise registration between fluorescently labeled mRNA, nuclear pore complexes, and chromatin, we obtained globally optimal image alignment within 80-nm precision using transformation models. We show that β-actin mRNAs freely access the entire nucleus and fewer than 60\% of mRNAs are more than 0.5 {\textmu}m away from a nuclear pore, and we do so for the first time accounting for spatial inhomogeneity of nuclear organization.</p>
},
	issn = {1540-8140},
	doi = {10.1083/jcb.201411032},
	author = {Smith, Carlas S and Preibisch, Stephan and Joseph, Aviva and Abrahamsson, Sara and Rieger, Bernd and Myers, Eugene and Singer, Robert H and Grunwald, David}
}
