@article {65273,
	title = {Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages.},
	journal = {eLife},
	volume = {9},
	year = {2020},
	month = {2020 Jan 08},
	abstract = {<p>Inner ear cochlear spiral ganglion neurons (SGNs) transmit auditory information to the brainstem. Recent single cell RNA-Seq studies have revealed heterogeneities within SGNs. Nonetheless, much remains unknown about the transcriptome of SGNs, especially which genes are specifically expressed in SGNs. To address these questions we needed a deeper and broader gene coverage than that in previous studies. We performed bulk RNA-Seq on mouse SGNs at five ages, and on two reference cell types (hair cells and glia). Their transcriptome comparison identified genes previously unknown to be specifically expressed in SGNs. To validate our dataset and provide useful genetic tools for this research field, we generated two knockin mouse strains: and . Our comprehensive analysis confirmed the SGN-selective expression of the candidate genes, testifying to the quality of our transcriptome data. These two mouse strains can be used to temporally label SGNs or to sort them.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.50491},
	author = {Li, Chao and Li, Xiang and Bi, Zhenghong and Sugino, Ken and Wang, Guangqin and Zhu, Tong and Liu, Zhiyong}
}
@article {47849,
	title = {Cell class-lineage analysis reveals sexually dimorphic lineage compositions in the Drosophila brain.},
	journal = {Current Biology : CB},
	volume = {26},
	year = {2016},
	month = {2016 Sep 08},
	pages = {2583-93},
	abstract = {<p>The morphology and physiology of neurons are directed by developmental decisions made within their lines of descent from single stem cells. Distinct stem cells may produce neurons having shared properties that define their cell class, such as the type of secreted neurotransmitter. The relationship between cell class and lineage is complex. Here we developed the transgenic cell class-lineage intersection (CLIn) system to assign cells of a particular class to specific lineages within the Drosophila brain. CLIn also enables birth-order analysis and genetic manipulation of particular cell classes arising from particular lineages. We demonstrated the power of CLIn in the context of the eight central brain type II lineages, which produce highly diverse progeny through intermediate neural progenitors. We mapped 18 dopaminergic neurons from three distinct clusters to six type\&nbsp;II lineages that show lineage-characteristic neurite trajectories. In addition, morphologically distinct dopaminergic neurons are produced within a given lineage, and they arise in an invariant sequence. We also identified type II lineages that produce doublesex- and fruitless-expressing neurons and examined whether female-specific apoptosis in these lineages accounts for the lower number of these neurons in the female brain. Blocking apoptosis in these lineages resulted in more cells in both sexes with males still carrying more cells than females. This argues that sex-specific stem cell fate together with differential progeny apoptosis contribute to the final sexual dimorphism.</p>
},
	issn = {1879-0445},
	doi = {10.1016/j.cub.2016.07.086},
	author = {Ren, Qingzhong and Awasaki, Takeshi and Huang, Yu-Fen and Liu, Zhiyong and Lee, Tzumin}
}
