@article {47467,
	title = {Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.},
	journal = {Nature Cell Biology},
	volume = {17},
	year = {2015},
	month = {2015 Feb 16},
	pages = {228-40},
	abstract = {<p>Membrane association with mother centriole (M-centriole) distal appendages is critical for ciliogenesis initiation. How the Rab GTPase Rab11{\textendash}​Rab8\&nbsp;cascade functions in early ciliary membrane assembly is unknown. Here, we show that the membrane shaping proteins ​EHD1\&nbsp;and ​EHD3, in association with the Rab11{\textendash}​Rab8\&nbsp;cascade, function in early ciliogenesis. ​EHD1\&nbsp;and ​EHD3\&nbsp;localize to preciliary membranes and the ciliary pocket. EHD-dependent membrane tubulation is essential for ciliary vesicle formation from smaller distal appendage vesicles (DAVs). Importantly, this step functions in M-centriole to basal body transformation and recruitment of transition zone proteins and ​IFT20. ​SNAP29, a SNARE membrane fusion regulator and ​EHD1-binding protein, is also required for DAV-mediated ciliary vesicle assembly. Interestingly, only after ciliary vesicle assembly is ​Rab8\&nbsp;activated for ciliary growth. Our studies uncover molecular mechanisms informing a previously uncharacterized ciliogenesis step, whereby ​EHD1\&nbsp;and ​EHD3\&nbsp;reorganize the M-centriole and associated DAVs before coordinated ciliary membrane and axoneme growth.</p>
},
	issn = {1476-4679},
	doi = {10.1038/ncb3109},
	author = {Lu, Quanlong and Insinna, Christine and Ott, Carolyn and Stauffer, Jimmy and Pintado, Petra A and Rahajeng, Juliati and Baxa, Ulrich and Walia, Vijay and Cuenca, Adrian and Hwang, Yoo-Seok and Daar, Ira O and Lopes, Susana and Lippincott-Schwartz, Jennifer and Jackson, Peter K and Caplan, Steve and Westlake, Christopher J}
}
