@article {49405,
	title = {MicroED structure of the NaK ion channel reveals a Na partition process into the selectivity filter.},
	journal = {Communications Biology},
	volume = {1},
	year = {2018},
	month = {2018},
	pages = {38},
	abstract = {<p>Sodium (Na) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration states of Na are proposed to play critical roles in determining the conductance and the selectivity of Na channels, yet they are rarely captured by conventional structural biology means. Here we use the emerging cryo-electron microscopy (cryoEM) method micro-electron diffraction (MicroED) to study the structure of a prototypical tetrameric Na-conducting channel, NaK, to 2.5 {\r A} resolution from nano-crystals. Two new conformations at the external site of NaK are identified, allowing us to visualize a partially hydrated Na ion at the entrance of the channel pore. A process of dilation coupled with Na movement is identified leading to valuable insights into the mechanism of ion conduction and gating. This study lays the ground work for future studies using MicroED in membrane protein biophysics.</p>
},
	issn = {2399-3642},
	doi = {10.1038/s42003-018-0040-8},
	author = {Liu, Shian and Gonen, Tamir}
}
@article {47730,
	title = {Atomic resolution structure determination by the cryo-EM method MicroED.},
	journal = {Protein Science : a Publication of the Protein Society},
	volume = {26},
	year = {2016},
	month = {2016 Jul 25},
	pages = {8-15},
	abstract = {<p>The electron cryo-microscopy (cryoEM) method MicroED has been rapidly developing. In this review we highlight some of the key steps in MicroED from crystal analysis to structure determination. We compare and contrast MicroED and the latest X-ray based diffraction method the X-ray free electron laser (XFEL). Strengths and shortcomings of both MicroED and XFEL are discussed. Finally, all current MicroED structures are tabulated with a view to the future. This article is protected by copyright. All rights reserved.</p>
},
	issn = {1469-896X},
	doi = {10.1002/pro.2989},
	author = {Liu, Shian and Hattne, Johan and Reyes, Francis E and Sanchez-Martinez, Silvia and de la Cruz, M Jason and Shi, D and Gonen, T}
}
