@article {49455,
	title = {moxMaple3: a photoswitchable fluorescent protein for PALM and protein highlighting in oxidizing cellular environments.},
	journal = {Scientific Reports},
	volume = {8},
	year = {2018},
	month = {2018 Oct 03},
	pages = {14738},
	abstract = {<p>The ability of fluorescent proteins (FPs) to fold robustly is fundamental to the autocatalytic formation of the chromophore. While the importance of the tertiary protein structure is well appreciated, the impact of individual amino acid mutations for FPs is often not intuitive and requires direct testing. In this study, we describe the engineering of a monomeric photoswitchable FP, moxMaple3, for use in oxidizing cellular environments, especially the eukaryotic secretory pathway. Surprisingly, a point mutation to replace a cysteine substantially improved the yield of correctly folded FP capable of chromophore formation, regardless of cellular environment. The improved folding of moxMaple3 increases the fraction of visibly tagged fusion proteins, as well as FP performance in PALM super-resolution microscopy, and thus makes moxMaple3 a robust monomeric FP choice for PALM and optical highlighting applications.</p>
},
	issn = {2045-2322},
	doi = {10.1038/s41598-018-32955-5},
	author = {Kaberniuk, Andrii A and Mohr, Manuel A and Verkhusha, Vladislav V and Snapp, Erik Lee}
}
@article {48223,
	title = {moxDendra2: an inert photoswitchable protein for oxidizing environments.},
	journal = {Chemical Communications},
	volume = {53},
	year = {2017},
	month = {2017 Jan 19},
	pages = {2106-9},
	abstract = {<p>Fluorescent proteins (FPs) that can be optically highlighted enable PALM super-resolution microscopy and pulse-chase experiments of cellular molecules. Most FPs evolved in cytoplasmic environments either in the original source organism or in the cytoplasm of bacteria during the course of optimization for research applications. Consequently, many FPs may fold incorrectly in the chemically distinct environments in subcellular organelles. Here, we describe the first monomeric photoswitchable (from green to bright red) FP adapted for oxidizing environments.</p>
},
	doi = {10.1039/C6CC09997A},
	author = {Kaberniuk, Andrii A and Morano, Nicholas C and Verkhusha, Vladislav V and Snapp, Erik Lee}
}
