@article {6274,
	title = {Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission.},
	journal = {Molecular Biology of the Cell},
	volume = {24},
	year = {2013},
	month = {2013 Aug},
	pages = {2319-27},
	abstract = {<p>The endosomal-sorting complex required for transport (ESCRT) is evolutionarily conserved from Archaea to eukaryotes. The complex drives membrane scission events in a range of processes, including cytokinesis in Metazoa and some Archaea. CdvA is the protein in Archaea that recruits ESCRT-III to the membrane. Using electron cryotomography (ECT), we find that CdvA polymerizes into helical filaments wrapped around liposomes. ESCRT-III proteins are responsible for the cinching of membranes and have been shown to assemble into helical tubes in vitro, but here we show that they also can form nested tubes and nested cones, which reveal surprisingly numerous and versatile contacts. To observe the ESCRT-CdvA complex in a physiological context, we used ECT to image the archaeon Sulfolobus acidocaldarius and observed a distinct protein belt at the leading edge of constriction furrows in dividing cells. The known dimensions of ESCRT-III proteins constrain their possible orientations within each of these structures and point to the involvement of spiraling filaments in membrane scission.</p>
},
	issn = {1939-4586},
	doi = {10.1091/mbc.E12-11-0785},
	author = {Dobro, Megan J and Samson, Rachel Y and Yu, Zhiheng and McCullough, John and Ding, H Jane and Chong, Parkson Lee-Gau and Bell, Stephen D and Jensen, Grant J}
}
