@article {65757,
	title = {Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin.},
	journal = {Science Advances},
	volume = {6},
	year = {2020},
	month = {2020 Sep 23},
	abstract = {<p>Three-dimensional (3D) chromatin organization plays a key role in regulating mammalian genome function; however, many of its physical features at the single-cell level remain underexplored. Here, we use live- and fixed-cell 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify chains of interlinked ~200- to 300-nm-wide chromatin domains (CDs) composed of aggregated nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin compartment. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications toward the core of CDs in both hetero- and euchromatic regions. This nanoscale functional topography is temporarily relaxed in postreplicative chromatin but remarkably persists after ablation of cohesin. Our findings establish CDs as physical and functional modules of mesoscale genome organization.</p>
},
	issn = {2375-2548},
	doi = {10.1126/sciadv.aba8811},
	author = {Miron, Ezequiel and Oldenkamp, Roel and Brown, Jill M and Pinto, David M S and Xu, C Shan and Faria, Ana R and Shaban, Haitham A and Rhodes, James D P and Innocent, Cassandravictoria and de Ornellas, Sara and Hess, Harald F and Buckle, Veronica and Schermelleh, Lothar}
}
@article {48809,
	title = {Cohesin can remain associated with chromosomes during DNA replication.},
	journal = {Cell Reports},
	volume = {20},
	year = {2017},
	month = {2017 Sep 19},
	pages = {2749-55},
	abstract = {<p>To ensure disjunction to opposite poles during anaphase, sister chromatids must be held together following DNA replication. This is mediated by cohesin, which is thought to entrap sister DNAs inside a tripartite ring composed of its Smc and kleisin (Scc1) subunits. How such structures are created during S phase is poorly understood, in particular whether they are derived from complexes that had entrapped DNAs prior to replication. To address this, we used selective photobleaching to determine whether cohesin associated with chromatin in G1 persists in\&nbsp;situ after replication. We developed a non-fluorescent HaloTag ligand to discriminate the fluorescence recovery signal from labeling of newly synthesized Halo-tagged Scc1 protein (pulse-chase or pcFRAP). In cells where cohesin turnover is inactivated by deletion of WAPL, Scc1 can remain associated with chromatin throughout S phase. These findings suggest that cohesion might be generated by cohesin that is already bound to un-replicated DNA.</p>
},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2017.08.092},
	author = {Rhodes, James D P and Haarhuis, Judith H I and Grimm, Jonathan B and Rowland, Benjamin D and Lavis, Luke D and Nasmyth, Kim A}
}
