@article {48936,
	title = {A moving source of matrix components is essential for de novo basement membrane formation.},
	journal = {Current Biology : CB},
	volume = {27},
	year = {2017},
	month = {2017 Nov 02},
	pages = {3526-34},
	abstract = {<p>The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is unknown how all of these components are generated and subsequently constructed to form a fully mature BM in the living animal. Although BM formation is thought to simply involve a process of self-assembly [2], this concept suffers from a number of logistical issues when considering its construction in\&nbsp;vivo. First, incorporation of BM components appears to be hierarchical [3-5], yet it is unclear whether their production during embryogenesis must also be regulated in a temporal fashion. Second, many BM proteins are produced not only by the cells residing on the BM but also by surrounding cell types [6-9], and it is unclear how large, possibly insoluble protein complexes [10] are delivered into the matrix. Here we exploit our ability to live image and genetically dissect de novo BM formation during Drosophila development. This reveals that there is a temporal hierarchy of BM protein production that is essential for proper component incorporation. Furthermore, we show that BM components require secretion by migrating macrophages (hemocytes) during their developmental dispersal, which is critical for embryogenesis. Indeed, hemocyte migration is essential to deliver a subset of ECM components evenly throughout the embryo. This reveals that de novo BM construction requires a combination of both production and distribution logistics allowing for the timely delivery of core components.</p>
},
	issn = {1879-0445},
	doi = {10.1016/j.cub.2017.10.001},
	author = {Matsubayashi, Yutaka and Louani, Adam and Dragu, Anca and S{\'a}nchez-S{\'a}nchez, Besaiz J and Serna-Morales, Eduardo and Yolland, Lawrence and Gyoergy, Attila and Vizcay, Gema and Fleck, Roland A and Heddleston, John M and Chew, Teng-Leong and Siekhaus, Daria E and Stramer, Brian M}
}
