@article {67763, title = {An adaptive optics module for deep tissue multiphoton imaging in vivo.}, journal = {Nature Methods}, volume = {18}, year = {2021}, month = {2021 Oct 01}, pages = {1259-1264}, abstract = {

Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.

}, issn = {1548-7105}, doi = {10.1038/s41592-021-01279-0}, author = {Rodriguez, Cristina and Chen, Anderson and Rivera, Jos{\'e} A and Mohr, Manuel A and Liang, Yajie and Natan, Ryan G and Sun, Wenzhi and Milkie, Daniel E and Bifano, Thomas G and Chen, Xiaoke and Ji, Na} } @article {49031, title = {Adaptive optical microscopy for neurobiology.}, journal = {Current Opinion in Neurobiology}, volume = {50}, year = {2018}, month = {2018 Jun}, pages = {83-91}, abstract = {

Highlights:

With the ability to correct for the aberrations introduced by biological specimens, adaptive optics{\textemdash}a method originally developed for astronomical telescopes{\textemdash}has been applied to optical microscopy to recover diffraction-limited imaging performance deep within living tissue. In particular, this technology has been used to improve image quality and provide a more accurate characterization of both structure and function of neurons in a variety of living organisms. Among its many highlights, adaptive optical microscopy has made it possible to image large volumes with diffraction-limited resolution in zebrafish larval brains, to resolve dendritic spines over 600μm deep in the mouse brain, and to more accurately characterize the orientation tuning properties of thalamic boutons in the primary visual cortex of awake mice.

}, doi = {10.1016/j.conb.2018.01.011}, author = {Rodriguez, Cristina and Ji, Na} } @article {49181, title = {Three-photon fluorescence microscopy with an axially elongated Bessel focus.}, journal = {Optics Letters}, volume = {43}, year = {2018}, month = {2018 Apr 15}, pages = {1914-1917}, abstract = {

Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in the field of neuroscience, where the ability to image neurons and their networks with high spatiotemporal resolution is essential. Using an axially elongated focus approximating a Bessel beam, in combination with two-photon fluorescence microscopy, has proven successful at such an endeavor. Here, we demonstrate three-photon fluorescence imaging with an axially extended Bessel focus. We use an axicon-based module that allowed for the generation of Bessel foci of varying numerical apertures and axial lengths, and apply this volumetric imaging tool to image mouse brain slices and for in vivo imaging of the mouse brain.

}, issn = {1539-4794}, doi = {10.1364/OL.43.001914}, author = {Rodriguez, Cristina and Liang, Yajie and Lu, Rongwen and Ji, Na} }