@article {49456,
	title = {A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels.},
	journal = {Scientific Reports},
	volume = {8},
	year = {2018},
	month = {2018 Oct 03},
	pages = {14768},
	abstract = {<p>Maintenance of the bacterial homeostasis initially emanates from interactions between proteins and the bacterial nucleoid. Investigating their spatial correlation requires high spatial resolution, especially in tiny, highly confined and crowded bacterial cells. Here, we present super-resolution microscopy using a palette of fluorescent labels that bind transiently to either the membrane or the nucleoid of fixed E. coli cells. The presented labels are easily applicable, versatile and allow long-term single-molecule super-resolution imaging independent of photobleaching. The different spectral properties allow for multiplexed imaging in combination with other localisation-based super-resolution imaging techniques. As examples for applications, we demonstrate correlated super-resolution imaging of the bacterial nucleoid with the position of genetic loci, of nascent DNA in correlation to the entire nucleoid, and of the nucleoid of metabolically arrested cells. We furthermore show that DNA- and membrane-targeting labels can be combined with photoactivatable fluorescent proteins and visualise the nano-scale distribution of RNA polymerase relative to the nucleoid in drug-treated E. coli cells.</p>
},
	issn = {2045-2322},
	doi = {10.1038/s41598-018-33052-3},
	author = {Spahn, Christoph K and Glaesmann, Mathilda and Grimm, Jonathan B and Ayala, Anthony X and Lavis, Luke D and Heilemann, Mike}
}
@article {49576,
	title = {Whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores.},
	journal = {Nano Letters},
	volume = {19},
	year = {2018},
	month = {2018 Dec 10},
	pages = {500-5},
	abstract = {<p>We demonstrate STED microscopy of whole bacterial and eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging. We achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy.</p>
},
	issn = {1530-6992},
	doi = {10.1021/acs.nanolett.8b04385},
	author = {Spahn, Christoph and Grimm, Jonathan B and Lavis, Luke D and Lampe, Marko and Heilemann, Mike}
}
