@article {65998,
	title = {A transcriptomic taxonomy of  circadian neurons around the clock.},
	journal = {eLife},
	volume = {10},
	year = {2021},
	month = {2021 Jan 13},
	abstract = {<p>Many different functions are regulated by circadian rhythms, including those orchestrated by discrete clock neurons within animal brains. To comprehensively characterize and assign cell identity to the 75 pairs of circadian neurons, we optimized a single cell RNA sequencing method and assayed clock neuron gene expression at different times of day. The data identify at least 17 clock neuron categories with striking spatial regulation of gene expression. Transcription factor regulation is prominent and likely contributes to the robust circadian oscillation of many transcripts, including those that encode cell-surface proteins previously shown to be important for cell recognition and synapse formation during development. The many other clock-regulated genes also constitute an important resource for future mechanistic and functional studies between clock neurons and/or for temporal signaling to circuits elsewhere in the fly brain.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.63056},
	author = {Ma, Dingbang and Przybylski, Dariusz and Abruzzi, Katharine C and Schlichting, Matthias and Li, Qunlong and Long, Xi and Rosbash, Michael}
}
@article {49572,
	title = {Allatostatin-C/AstC-R2 is a novel pathway to modulate the circadian activity pattern in Drosophila.},
	journal = {Current Biology : CB},
	volume = {29},
	year = {2018},
	month = {2018 Dec 04},
	pages = {13-22},
	abstract = {<p>Seven neuropeptides are expressed within the Drosophila brain circadian network. Our previous mRNA profiling suggested that Allatostatin-C (AstC) is an eighth neuropeptide and specifically expressed in dorsal clock neurons (DN1s). Our results here show that AstC is, indeed, expressed in DN1s, where it oscillates. AstC is also expressed in two less well-characterized circadian neuronal clusters, the DN3s and lateral-posterior neurons (LPNs). Behavioral experiments indicate that clock-neuron-derived AstC is required to mediate evening locomotor activity under short (winter-like) and long (summer-like) photoperiods. The AstC-Receptor 2 (AstC-R2) is expressed in LNds, the clock neurons that drive evening locomotor activity, and AstC-R2 is required in these neurons to modulate the same short photoperiod evening phenotype. Ex\&nbsp;vivo calcium imaging indicates that AstC directly inhibits a single LNd. The results suggest that a novel AstC/AstC-R2 signaling pathway, from dorsal circadian neurons to an LNd, regulates the evening phase in Drosophila.</p>
},
	issn = {1879-0445},
	doi = {10.1016/j.cub.2018.11.005},
	author = {D{\'\i}az, Madelen M and Schlichting, Matthias and Abruzzi, Katharine C and Long, Xi and Rosbash, Michael}
}
