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Viruses

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Grigorieff Lab / Viruses
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Many viruses form large assemblies that are highly symmetrical. They are therefore excellent particles for the development and testing of cryo-EM methods.
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Viruses
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Many viruses form large assemblies that are highly symmetrical. They are therefore excellent particles for the development and testing of cryo-EM methods.
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We are collaborating with Stephen Harrison (HHMI, Harvard Medical School) to use virus particles as test specimens to develop better single particle image-processing methods. Virus particles have a high degree of symmetry and are stable in an aqueous solution, making them ideal for EM imaging.

We have used rotavirus double-layer particles (DLPs) to determine a structure of several of the capsid proteins to a resolution of 3.8 Å. Rotavirus is also a good particle to investigate current limitations in cryo-EM, such as beam-induced motion, and to develop and test new image processing methods, such as motion correction.

Furthermore, we use helical viruses, such as Tobacco Mosaic Virus, to test new algorithms for helical particle processing.