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2 Publications

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    12/01/14 | Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology.
    Andrasfalvy BK, Galiñanes GL, Huber D, Barbic M, Macklin JJ, Susumu K, Delehanty JB, Huston AL, Makara JK, Medintz IL
    Nature Methods. 2014 Dec;11(12):1237-41. doi: 10.1038/nmeth.3146

    Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.

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    03/01/14 | Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals.
    Berenyi A, Somogyvári Z, Nagy AJ, Roux L, Long JD, Fujisawa S, Stark E, Leonardo A, Harris TD, Buzsáki G
    Journal of Neurophysiology. 2014 Mar;111(5):1132-49. doi: 10.1152/jn.00785.2013

    Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.

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