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3 Publications

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    10/01/06 | Local caspase activity directs engulfment of dendrites during pruning.
    Williams DW, Kondo S, Krzyzanowska A, Hiromi Y, Truman JW
    Nature Neuroscience. 2006 Oct;9(10):1234-6. doi: 10.1038/nn1774

    Pruning is important for sculpting neural circuits, as it removes excessive or inaccurate projections. Here we show that the removal of sensory neuron dendrites during pruning in Drosophila melanogaster is directed by local caspase activity. Suppressing caspase activity prevented dendrite removal, whereas a global activation of caspases within a neuron caused cell death. A new genetically encoded caspase probe revealed that caspase activity is confined to the degenerating dendrites of pruning neurons.

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    Truman LabRiddiford Lab
    06/02/06 | Juvenile hormone is required to couple imaginal disc formation with nutrition in insects.
    Truman JW, Hiruma K, Allee JP, Macwhinnie SG, Champlin DT, Riddiford LM
    Science . 2006 Jun 2;312(5778):1385-8. doi: 10.1126/science.1123652

    In starved larvae of the tobacco hornworm moth Manduca sexta, larval and imaginal tissues stop growing, the former because they lack nutrient-dependent signals but the latter because of suppression by juvenile hormone. Without juvenile hormone, imaginal discs form and grow despite severe starvation. This hormone inhibits the intrinsic signaling needed for disc morphogenesis and does so independently of ecdysteroid action. Starvation and juvenile hormone treatments allowed the separation of intrinsic and nutrient-dependent aspects of disc growth and showed that both aspects must occur during the early phases of disc morphogenesis to ensure normal growth leading to typical-sized adults.

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    Riddiford LabTruman Lab
    05/02/06 | The pupal specifier broad directs progressive morphogenesis in a direct-developing insect.
    Erezyilmaz DF, Riddiford LM, Truman JW
    Proceedings of the National Academy of Sciences of the United States of America. 2006 May 2;103:6925-30. doi: 10.1073/pnas.0509983103

    A key regulatory gene in metamorphosing (holometabolous) insect life histories is the transcription factor broad (br), which specifies pupal development. To determine the role of br in a direct-developing (hemimetabolous) insect that lacks a pupal stage, we cloned br from the milkweed bug, Oncopeltus fasciatus (Of’br). We find that, unlike metamorphosing insects, in which br expression is restricted to the larval-pupal transition, Of’br mRNA is expressed during embryonic development and is maintained at each nymphal molt but then disappears at the molt to the adult. Induction of a supernumerary nymphal stage with a juvenile hormone (JH) mimic prevented the disappearance of br mRNA. In contrast, induction of a precocious adult molt by application of precocene II to third-stage nymphs caused a loss of br mRNA at the precocious adult molt. Thus, JH is necessary to maintain br expression during the nymphal stages. Injection of Of’br dsRNA into either early third- or fourth-stage nymphs caused a repetition of stage-specific pigmentation patterns and prevented the normal anisometric growth of the wing pads without affecting isometric growth or molting. Therefore, br is necessary for the mutable (heteromorphic) changes that occur during hemimetabolous development. Our results suggest that metamorphosis in insects arose as expression of br, which conveys competence for change, became restricted to one postembryonic instar. After this shift in br expression, the progressive changes that occur within the nymphal series in basal insects became compressed to the one short period of morphogenesis seen in the larva-to-pupa transition of holometabolous insects.

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