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    Lavis LabTranscription Imaging
    05/03/16 | RNA Polymerase II cluster dynamics predict mRNA output in living cells.
    Cho W, Jayanth N, English BP, Inoue T, Andrews JO, Conway W, Grimm JB, Spille J, Lavis LD, Lionnet T, Cisse II
    eLife. 2016 May 03;5:. doi: 10.7554/eLife.13617

    Protein clustering is a hallmark of genome regulation in mammalian cells. However, the dynamic molecular processes involved make it difficult to correlate clustering with functional consequences in vivo. We developed a live-cell super-resolution approach to uncover the correlation between mRNA synthesis and the dynamics of RNA Polymerase II (Pol II) clusters at a gene locus. For endogenous β-actin genes in mouse embryonic fibroblasts, we observe that short-lived (~8 s) Pol II clusters correlate with basal mRNA output. During serum stimulation, a stereotyped increase in Pol II cluster lifetime correlates with a proportionate increase in the number of mRNAs synthesized. Our findings suggest that transient clustering of Pol II may constitute a pre-transcriptional regulatory event that predictably modulates nascent mRNA output.

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