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02/01/11 |
Histochemistry: live and in color.
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society. 2011 Feb;59:139-45. doi: 10.1369/0022155410395760
Histochemistry (chemistry in the context of biological tissue) is an invaluable set of techniques used to visualize biological structures. This field lies at the interface of organic chemistry, biochemistry, and biology. Integration of these disciplines over the past century has permitted the imaging of cells and tissues using microscopy. Today, by exploiting the unique chemical environments within cells, heterologous expression techniques, and enzymatic activity, histochemical methods can be used to visualize structures in living matter. This review focuses on the labeling techniques and organic fluorophores used in live cells.