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12/10/18 |
Whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores.
Nano Letters. 2018 Dec 10;19(1):500-5. doi: 10.1021/acs.nanolett.8b04385
We demonstrate STED microscopy of whole bacterial and eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging. We achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy.