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GCaMP calcium indicators

 

Plasmids available from Addgene.org :

 

Name Description Addgene Plasmid ID Reference

CMV-GCaMP5G

 

31788

Akerboom et al., 2012

CMV-GCaMP3

 

22692

Tian et al., 2009

pCMV-GCaMP6s

slow, sensitive

40753

Chen et al., 2013

pCMV-GCaMP6m

medium

40754

Chen et al., 2013

pCMV-GCaMP6f

fast

40755

Chen et al., 2013

 

Viruses available from University of Pennsylvania Viral Vector Core:

 

Name Penn Catalog Number Penn Map and Sequence Number Reference

AAV1.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-1-PV2478

Penn Vector P2478

Akerboom et al., 2013

AAV5.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-5-PV2478

Penn Vector P2478

Akerboom et al., 2013

AAV9.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2478

Penn Vector P2478

Akerboom et al., 2013

AAV9.CAG.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2643

Penn Vector P2643

Akerboom et al., 2013

AAV1.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-1-PV2541

Penn Vector P2541

Akerboom et al., 2013

AAV9.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2541

Penn Vector P2541

Akerboom et al., 2013

AAV1.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-1-PV2540

Penn Vector 2540

Akerboom et al., 2013

AAV5.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-5-PV2540

Penn Vector P2540

Akerboom et al., 2013

AAV9.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2540

Penn Vector P2540

Akerboom et al., 2013

AAV1.hSynap.GCaMP3.WPRE.SV40

AV-1-PV1627

Penn Vector P1627

Tian et al., 2013

AAV5.hSynap.GCaMP3.WPRE.SV40

AV-5-PV1627

Penn Vector P1627

Tian et al., 2013

AAV9.hSynap.GCaMP3.WPRE.SV40

AV-9-PV1627

Penn Vector P1627

Tian et al., 2013

AAV1.hSynap.Flex.GCaMP3.WPRE.SV40

AV-1-PV1921

Penn Vector P1921

Tian et al., 2013

AAV5.hSynap.Flex.GCaMP3.WPRE.SV40

AV-5-PV1921

Penn Vector P1921

Tian et al., 2013

AAV9.hSynap.Flex.GCaMP3.WPRE.SV40

AV-9-PV1921

Penn Vector P1921

Tian et al., 2013

AAV1.Syn.GCaMP6f.WPRE.SV40

AV-1-PV2822

Penn Vector P2822

Chen et al., 2013

AAV1.Syn.GCaMP6s.WPRE.SV40

AV-1-PV2824

Penn Vector P2824

Chen et al., 2013

AAV1.Syn.GCaMP6m.WPRE.SV40

AV-1-PV2823

Penn Vector P2823

Chen et al., 2013

AAV5.Syn.GCaMP6m.WPRE.SV40

AV-5-PV2823

Penn Vector P2823

Chen et al., 2013

AAV5.Syn.GCaMP6f.WPRE.SV40

AV-5-PV2822

Penn Vector P2822

Chen et al., 2013

AAV5.Syn.GCaMP6s.WPRE.SV40

AV-5-PV2824

Penn Vector P2824

Chen et al., 2013

AAV9.Syn.GCaMP6f.WPRE.SV40

AV-9-PV2822

Penn Vector P2822

Chen et al., 2013

AAV9.Syn.GCaMP6m.WPRE.SV40

AV-9-PV2823

Penn Vector P2823

Chen et al., 2013

AAV9.Syn.GCaMP6s.WPRE.SV40

AV-9-PV2824

Penn Vector P2824

Chen et al., 2013

AAV1.CAG.GCaMP6s.WPRE.SV40

AV-1-PV2833

Penn Vector P2833

Chen et al., 2013

AAV5.CAG.GCaMP6s.WPRE.SV40

AV-5-PV2833

Penn Vector P2833

Chen et al., 2013

AAV9.CAG.GCaMP6s.WPRE.SV40

AV-9-PV2833

Penn Vector P2833

Chen et al., 2013

AAV1.CAG.Flex.GCaMP6f.WPRE.SV40

AV-1-PV2816

Penn Vector 2816

Chen et al., 2013

AAV1.CAG.GCaMP6f.WPRE.SV40 

AV-1-PV3081

Penn Vector P3081

Chen et al., 2013

AAV9.CAG.GCaMP6m.WPRE.SV40

AV-5-PV3080

Penn Vector P3080

Chen et al., 2013

AAV9.CAG.GCaMP6f.WPRE.SV40

AV-9-PV3081

Penn Vector P3081

Chen et al., 2013

AAV1.hSyn.Flex.iGluSnFR.WPRE.SV40

AV-1-PV2724

Penn Vector P2724

Marvin et al., 2013

AAV5.hSyn.Flex.iGluSnFR.WPRE.SV40

AV-5-PV2724

Penn Vector P2724

Marvin et al., 2013

AAV9.hSyn.Flex.iGluSnFR.WPRE.SV40

AV-9-PV2724

Penn Vector P2724

Marvin et al., 2013

AAV1.CAG.Flex.iGluSnFR.WPRE.SV40

AV-1-PV2725

Penn Vector P2725

Marvin et al., 2013

AAV5.CAG.Flex.iGluSnFR.WPRE.SV40

AV-5-PV2725

Penn Vector P2725

Marvin et al., 2013

AAV9.CAG.Flex.iGluSnFR.WPRE.SV40

AV-9-PV2725

Penn Vector P2725

Marvin et al., 2013

 

Mice available from The Jackson Laboratory:

 

Name Jax Stock Number Reference

Rosa26-CAG-lox-stop-lox-GCaMP3-WPRE

014538

Zariwala et al., 2010

 

024275

 

 

 

024339

 

 

 

024276

 

 

 

Collaborators available at The Jackson Laboratory:

 

Name Jax Stock Number Reference

B6;129S6-Gt(ROSA)26Sortm96(CAG-GCaMP6s)Hze/J

024106

 

 

B6;129S-Igs7tm93.1(tetO-GCaMP6f)Hze/J

024103

 

 

Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm93.1(tetO-GCaMP6f)Hze/HzeJ

024107

 

 

B6;DBA-Tg(tetO-GCaMP6s)2Niell/J

024742

 

 

B6;129S-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/J

024105

 

 

B6;129S6-Polr2atm1(CAG-GCaMP5g,tdTomato)Tvrd/J

024477

 

 

 

Flies available from Bloomington Stock Center:

 

Line name Insertion Site Bloomington Stock Number Reference Chr
P{20XUAS-IVS-GCaMP5G} attP40 42037 Akerboom et al., 2012  2
P{20XUAS-IVS-GCaMP5G} VK00005 42038 Akerboom et al., 2012  3

P{UAS-GCaMP3.T}

attP40

32116

Tian et al., 2009

 2

P{20XUAS-GCaMP3} su(Hw)attP8 32234 Tian et al., 2009  1
P{20XUAS-GCaMP3} attP18 32235 Tian et al., 2009  1
P{20XUAS-GCaMP3} attP2 32236 Tian et al., 2009  3
PBac{20XUAS-GCaMP3} VK00005 32237 Tian et al., 2009  3
P{20XUAS-IVS-GCaMP6s} attP40 42746  Chen et al., 2013  2

P{20XUAS-IVS-GCaMP6f}

attP40

42747

Chen et al., 2013

 2

P{20XUAS-IVS-GCaMP6m}

attP40

42748

Chen et al., 2013

 2

PBac{20XUAS-IVS-GCaMP6s}

VK00005

42749  

Chen et al., 2013

 3

PBac{20XUAS-IVS-GCaMP6m}

VK00005

42750

Chen et al., 2013

 3

w[1118]; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-GCaMP6s-SV40}

su(Hw)attP1

44273

Chen et al., 2013

 3

w[1118]; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-GCaMP6s-p10}

su(Hw)attP1

44274

Chen et al., 2013

 3

w[1118]; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-GCaMP6m-p10}

su(Hw)attP1

44275

Chen et al., 2013

 3

P{y[+mDint2] w[+mC]=13XLexAop2-IVS-GCaMP6m-p10}

VK00005/TM3, Sb[1]

44276

Chen et al., 2013

 3

w[1118]; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-GCaMP6f-p10}

su(Hw)attP5

44277

Chen et al., 2013

 2

w[1118]; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-GCaMP6m-SV40}

su(Hw)attP1

44588

Chen et al., 2013

 3

w[1118]; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-GCaMP6s-SV40}

su(Hw)attP5

44589

Chen et al., 2013

 2

w[1118]; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-GCaMP6s-p10}

su(Hw)attP5

44590

Chen et al., 2013

 2

 

RCaMP calcium indicators

 

Plasmids available from Addgene.org:

 

Name Description Addgene Plasmid ID Reference

pRSET-RCaMP1h

 

42874

Akerboom et al., 2013

 

Viruses available from University of Pennsylvania Viral Vector Core:

 

Name Penn Catalog Number Penn Map and Sequence Number Reference
AAV1.Syn.RCaMP1h.WPRE.SV40 AV-1-PV3010  Penn Vector PV3010 Looger/HHMI
AAV9.Syn.RCaMP1h.WPRE.SV40 AV-9-PV3010  Penn Vector PV3010 Looger/HHMI

 

Flies available from Bloomington Stock Center:

 

Line name Insertion Site Bloomington Stock Number Reference Chr
pJFRC-7.20X RCaMP1f VK00005 52210 Akerboom et al., 2013  
w+ DL; pJFRC-7.20X RCaMP1f attP2 (DL) 51928 Akerboom et al., 2013  

w+ CS; pJFRC-7.20X RCaMP1f

attP40 (CS)

51929

Akerboom et al., 2013  
w+ DL; pJFRC-7.20X RCaMP1f attP40 (DL) 51930 Akerboom et al., 2013  

 

iGluSnFR glutamate indicators

 

Plasmids available from Addgene.org:

 

Name Description Addgene Plasmid ID Reference

pCMV-iGluSnFR

 

41732

Marvin et al., 2013

pRSET-iGluSnFR

 

41733

Marvin et al., 2013

 

Viruses available from University of Pennsylvania Viral Vector Core:

 

Name Penn Catalog Number Penn Map and Sequence Number Reference

AAV1.GFAP.iGluSnFr.WPRE.SV40

AV-1-PV2914

Penn Vector P2914

Marvin et al., 2013

AAV9.GFAP.iGluSnFr.WPRE.SV40

AV-9-PV2914

Penn Vector P2914

Marvin et al., 2013

AAV1.hSyn.iGluSnFr.WPRE.SV40

AV-1-PV2723

Penn Vector P2723

Marvin et al., 2013

AAV5.hSyn.iGluSnFr.WPRE.SV40

AV-5-PV2723

Penn Vector P2723

Marvin et al., 2013

AAV5.GFAP.iGluSnFr.WPRE.SV40

AV-5-PV2914

Penn Vector P2914

Marvin et al., 2013

AAV9.hSyn.iGluSnFr.WPRE.SV40

AV-9-PV2723

Penn Vector P2723

Marvin et al., 2013