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01/21/19 |
A genetically encoded near-infrared fluorescent calcium ion indicator.
Nature Methods. 2019 Jan 21;16(2):171-4. doi: 10.1038/s41592-018-0294-6
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca imaging in combination with other optogenetic indicators and actuators.