Gandhi S, Zenklusen D, Lionnet T, Singer RH.
Nat Struct Mol Biol 18(1):27-34 (2011 January). PMCID: PMC3058351
Correlation between transcripts from two alleles of a constitutively active gene, MDN1, in diploid cells.
Expression of an individual gene can vary considerably among genetically identical cells because of stochastic fluctuations in transcription. However, proteins comprising essential complexes or pathways have similar abundances and lower variability. It is not known whether coordination in the expression of subunits of essential complexes occurs at the level of transcription, mRNA abundance or protein expression. To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells. Our results revealed that transcript levels of temporally induced genes are highly correlated in individual cells. In contrast, transcription of constitutive genes encoding essential subunits of complexes is not coordinated because of stochastic fluctuations. The coordination of these functional complexes therefore must occur post- transcriptionally, and likely post-translationally.
Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH.
Science 332(6028):475-478 (2011 April 22). PMCID: PMC3152976
Real-time measurement of fluorescent RNA reveals transcription kinetics.
Grünwald D, Singer RH.
Nature 467(7315):604-607 (2010 September 30). PMCID: PMC3005609
Super-registration precision and detection of nuclear mRNA.
Localization & Translational Regulation from
Patel VL, Mitra S, Harris R, Buxbaum AR, Lionnet T, Brenowitz M, Girvin M, Levy M, Almo SC, Singer RH, Chao JA.
Genes Dev 26(1):43-53 (2012 January 1). PMCID: PMC3258965
The ZBP1 KH34 REs are required for distal dendritic RNA transport.
Trcek T, Larson DR, Moldón A, Query CC, Singer RH.
Cell 147(7):1484-1497 (2011 December 23).
Measuring mRNA Decay Rates Using Single-Cell, Single-Molecule FISH.
Regent Development from
Lionnet T, Czaplinski K, Darzacq X, Shav-Tal Y, Wells AL, Chao JA, Park HY, de Turris V, Lopez-Jones M, Singer RH.
Nat Methods 8(2):165-170 (2011 February). PMCID: PMC3076588
Schematic of the Actb-MS2 system for live-cell imaging.
Microscopy Development from
Grünwald D, Shenoy SM, Burke S, Singer RH.
Nat Protoc 3(11):1809-1814 (2008 November). PMCID: PMC3109976
Beam profile along the optical axis of the microscope.
Imaging the Brain While Forming Memories
Credit: Albert Einstein College of Medicine of Yeshiva University and Hye Yoon Park, Ph.D.