Carl Wu is interested in the organization and dynamics of chromatin and how chromatin compaction controls the functional activities of eukaryotic genomes.
Carl Wu continues to develop a broad range of biochemical methods designed to probe key features of chromatin structure common to all eukaryotes. At Janelia, he is working collaboratively with colleagues using advanced microscopy to image histone and chromatin dynamics in vitro and in live cells.
Nucleosome-free, DNase I hypersensitive promoters
Chromatin is the complex of DNA, histone proteins and associated macromolecules that not only packages eukaryotic genomes within the confines of the cell nucleus but also physically masks the sequence of DNA bases in the double helix, thereby preventing indiscriminate access to genetic information. This organization plays a key role in controlling genome activities throughout life, from the onset of embryonic development to cell, tissue and organ differentiation. Aside from its fundamental importance, chromatin is causally implicated in gene misregulation in many human diseases, including cancer.
The organization of chromatin starts at the level of nucleosomes, the primary histone-DNA units of compaction, through intermediate levels of folding of nucleosome arrays to the most compacted chromosome forms visible under the light microscope. However, at cis-regulatory DNA elements, chromatin folding is interrupted by structural changes in nucleosome organization linked to early steps of gene expression. Initial studies from our laboratory suggested that creation of such a poised chromatin state, revealed by DNase I hypersensitivity, is necessary for subsequent induction of transcription. Mapping of DNase I hypersensitivity has been a longstanding assay in the chromatin toolbox, enabling discoveries of long distance enhancers and locus control regions, and has evolved into current genome-wide protocols to detect nucleosome-free and nucleosome-depleted sites at cis-regulatory elements throughout eukaryotic genomes.
ATP-dependent chromatin remodeling enzymes
We also developed an exonuclease protection assay that revealed the first in vivo footprints of eukaryotic transcription factors, leading to purification by DNA affinity chromatography and characterization of the heat shock transcription factor HSF, which undergoes heat shock-induced trimerization and high affinity DNA binding. Subsequently, development of a DNase I hypersensitivity reconstitution assay led to the discovery that promoter-specific chromatin remodeling requires concerted actions of sequence-specific GAGA factor and a novel ATP-dependent enzyme activity in Drosophila extracts, named NURF (Nucleosome Remodeling Factor). Unbiased, multi-step biochemical purification of NURF identified the four-subunit complex and its catalytic component ISWI, related to the SWI/SNF ATPase previously identified genetically as a co-regulator of transcription. NURF repositions nucleosomes by ATP-dependent ‘sliding’ of histone octamers on DNA, and additional genetic and molecular studies showed its requirement for activation or repression of several hundred genes, and its essential function in embryonic and post-embryonic fly and mouse development.
Histone H2A.Z replacement at promoters
Currently we are studying budding yeast SWR1, a 14-subunit enzyme whose catalytic ATPase is related to ISWI and SWI/SNF. The conserved SWR1 complex remodels nucleosomes by a mechanism distinct from all other chromatin remodelers. SWR1 evicts a histone H2A-H2B dimer from a conventional nucleosome and replaces it with histone variant H2A.Z-H2B. Histone H2A.Z is highly conserved from yeast to human, is universally localized to eukaryotic promoters and enhancers, and is also implicated in transcription. To elucidate the structural and biochemical basis of enzyme activity, we are systematically dissecting the histone H2A.Z replacement reaction. We have found that histone replacement occurs uni-directionally from H2A to H2A.Z in a step-wise fashion, one dimer at a time, and is dependent on activation of the Swr1 ATPase by its two natural substrates, the conventional nucleosome and the H2A.Z-H2B dimer. Ongoing studies probe other steps in the histone H2A.Z exchange pathway, and their relationship and dynamic interplay with the assembly and function of the transcription machinery
We are also investigating the centromere-specific histone variant, CenH3, which forms the foundation of the kinetochore connecting daughter chromosomes to the spindle apparatus for faithful segregation of duplicated genomes. We have recently identified a yeast CenH3-specfic histone chaperone, Scm3, and are studying the assembly and maintenance of the centromere-specific nucleosome. We are particularly interested in how this unique variant nucleosome performs its specialized function as the key link to kinetochore proteins during chromosome segregation.