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    Collaboration projects:

  1. Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes. Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM. Nature. 2018 Apr 5;556(7699):64-69.
  2. Cryo-EM structure of an essential Plasmodium vivax invasion complex. Gruszczyk J, Huang RK, Chan LJ, Menant S, Hong C, Murphy JM, Mok YF, Griffin MDW, Pearson RD, Wong W, Cowman AF, Yu Z, Tham WH. Nature. 2018. doi: 10.1038/s41586-018-0249-1.
  3. Near-atomic resolution cryoelectron microscopy structure of the 30-fold homooligomeric SpoIIIAG channel essential to spore formation in Bacillus subtilis. Zeytuni N, Hong C, Flanagan KA, Worrall LJ, Theiltges KA, Vuckovic M, Huang RK, Massoni SC, Camp AH, Yu Z, Strynadka NC. Proc Natl Acad Sci U S A. 2017. pii: 201704310.
  4. Structural basis of bacterial transcription activation. Liu B, Hong C, Huang RK, Yu Z, Steitz TA. Science. 2017;358(6365):947-951
  5. Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body. Worrall LJ, Hong C, Vuckovic M, Deng W, Bergeron JR, Majewski DD, Huang RK, Spreter T, Finlay BB, Yu Z, Strynadka NC. Nature. 2016; 540:597-601.


Representative publications with data obtained at the facility:

  1. Molecular structure of human P-glycoprotein in the ATP-bound, outward-facing conformation. Kim Y, Chen J. Science. 2018 Feb 23;359(6378):915-919.
  2. Integrative structure and functional anatomy of a nuclear pore complex. Kim SJ, Fernandez-Martinez J, Nudelman I, Shi Y, Zhang W, Raveh B, Herricks T, Slaughter BD, Hogan JA, Upla P, Chemmama IE, Pellarin R, Echeverria I, Shivaraju M, Chaudhury AS, Wang J, Williams R, Unruh JR, Greenberg CH, Jacobs EY, Yu Z, de la Cruz MJ, Mironska R, Stokes DL, Aitchison JD, Jarrold MF, Gerton JL, Ludtke SJ, Akey CW, Chait BT, Sali A, Rout MP.  Nature. 2018 Mar 22;555(7697):475-482.
  3. Structural Mechanism of Functional Modulation by Gene Splicing in NMDA Receptors. Regan MC, Grant T, McDaniel MJ, Karakas E, Zhang J, Traynelis SF, Grigorieff N, Furukawa H. Neuron. 2018 May 2;98(3):521-529.e3.
  4. Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule. Tsai JC, Miller-Vedam LE, Anand AA, Jaishankar P, Nguyen HC, Renslo AR, Frost A, Walter P. Science. 2018 Mar 30;359(6383).
  5. Physical basis of amyloid fibril polymorphism. Close W, Neumann M, Schmidt A, Hora M, Annamalai K, Schmidt M, Reif B, Schmidt V, Grigorieff N, Fändrich M. Nat Commun. 2018 Feb 16;9(1):699.
  6. Cryo-EM structures of the TMEM16A calcium-activated chloride channel. Dang S, Feng S, Tien J, Peters CJ, Bulkley D, Lolicato M, Zhao J, Zuberbühler K, Ye W, Qi L, Chen T, Craik CS, Jan YN, Minor DL Jr, Cheng Y, Jan LY. Nature. 2017 Dec 21;552(7685):426-429.
  7. Decoding the centromeric nucleosome through CENP-N. Pentakota S, Zhou K, Smith C, Maffini S, Petrovic A, Morgan GP, Weir JR, Vetter IR, Musacchio A, Luger K. Elife. 2017 Dec 27;6. pii: e33442
  8. Structure of RNA polymerase bound to ribosomal 30S subunit.  Demo G, Rasouly A, Vasilyev N, Svetlov V, Loveland AB, Diaz-Avalos R, Grigorieff N, Nudler E, Korostelev AA. Elife. 2017 Oct 13;6. pii: e28560.
  9. The structural basis of flagellin detection by NAIP5: A strategy to limit pathogen immune evasion.  Tenthorey JL, Haloupek N, López-Blanco JR, Grob P, Adamson E, Hartenian E, Lind NA, Bourgeois NM, Chacón P, Nogales E, Vance RE. Science. 2017;358(6365):888-893.
  10. Architectures of Lipid Transport Systems for the Bacterial Outer Membrane. Ekiert DC, Bhabha G, Isom GL, Greenan G, Ovchinnikov S, Henderson IR, Cox JS, Vale RD. Cell. 2017;169(2):273-285.
  11. Ensemble cryo-EM elucidates the mechanism of translation fidelity. Loveland AB, Demo G, Grigorieff N, Korostelev AA. Nature. 2017;546(7656):113-117
  12. Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3. Schoebel S, Mi W, Stein A, Ovchinnikov S, Pavlovicz R, DiMaio F, Baker D, Chambers MG, Su H, Li D, Rapoport TA, Liao M. Nature. 2017;548(7667):352-355.
  13. Disabling Cas9 by an anti-CRISPR DNA mimic. Shin J, Jiang F, Liu JJ, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA. Sci Adv. 2017;3(7):e1701620.
  14. Structural Bases of Desensitization in AMPA Receptor-Auxiliary Subunit Complexes.Twomey EC, Yelshanskaya MV, Grassucci RA, Frank J, Sobolevsky AI. Neuron. 2017; 94(3):569-580
  15. Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones. Zhou CY, Stoddard CI, Johnston JB, Trnka MJ, Echeverria I, Palovcak E, Sali A, Burlingame AL, Cheng Y, Narlikar GJ. Cell Rep. 2017;19(10):2033-2044.
  16. Asymmetric recognition of HIV-1 Envelope trimer by V1V2 loop-targeting antibodies. Wang H, Gristick HB, Scharf L, West AP, Galimidi RP, Seaman MS, Freund NT, Nussenzweig MC, Bjorkman PJ. Elife. 2017;6. pii: e27389
  17. Electron cryo-microscopy structure of the mechanotransduction channel NOMPC. Jin P, Bulkley D, Guo Y, Zhang W, Guo Z, Huynh W, Wu S, Meltzer S, Cheng T, Jan LY, Jan YN, Cheng Y. Nature. 2017 Jul 6;547(7661):118-122.
  18. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H. Nat Struct Mol Biol. 2017;24(3):316-324
  19. Single-protein detection in crowded molecular environments in cryo-EM images. Rickgauer JP, Grigorieff N, Denk W. Elife. 2017;6. pii: e25648.
  20. Structure of the active form of human origin recognition complex and its ATPase motor module. Tocilj A, On KF, Yuan Z, Sun J, Elkayam E, Li H, Stillman B, Joshua-Tor L. Elife. 2017;6. pii: e20818.
  21. Structural Titration of Slo2.2, a Na+-Dependent K+ Channel. Hite RK, MacKinnon R. Cell. 2017;168(3):390-399.
  22. Structural basis of cooperativity in kinesin revealed by 3D reconstruction of a two-head-bound state on microtubules. Liu D, Liu X, Shang Z, Sindelar CV. Elife. 2017;6. pii: e24490.
  23. Cryo-electron tomography reveals novel features of a viral RNA replication compartment. Ertel KJ, Benefield D, Castaño-Diez D, Pennington JG, Horswill M, den Boon JA, Otegui MS, Ahlquist P. Elife. 2017;6. pii: e25940.
  24. CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface. Liu Y, Pan J, Jenni S, Raymond DD, Caradonna T, Do KT, Schmidt AG, Harrison SC, Grigorieff N. J Mol Biol. 2017;429(12):1829-1839.
  25. Conformational States of a Soluble, Uncleaved HIV-1 Envelope Trimer. Liu Y, Pan J, Cai Y, Grigorieff N, Harrison SC, Chen B. J Virol. 2017;91(10). pii: e00175-17.
  26. Atomic Structure of the Cystic Fibrosis Transmembrane Conductance Regulator. Zhang Z, Chen J. Cell. 2016 Dec 1;167(6):1586-1597.
  27. Structural basis for gating the high-conductance Ca2+-activated K+ channel. Hite RK, Tao X, MacKinnon R. Nature. 2016 Dec 14. doi: 10.1038/nature20775.
  28. Structure of the transporter associated with antigen processing trapped by herpes simplex virus. Oldham ML, Grigorieff N, Chen J. Elife. 2016 Dec 9;5. pii: e21829. doi: 10.7554/eLife.21829.
  29. A near atomic structure of the active human apoptosome. Cheng TC, Hong C, Akey IV, Yuan S, Akey CW. Elife. 2016 Oct 4;5. pii: e17755.
  30. A Near-Atomic Structure of the Dark Apoptosome Provides Insight into Assembly and Activation. Cheng TC, Akey IV, Yuan S, Yu Z, Ludtke SJ, Akey CW. Structure. 2016 Nov 16. pii: S0969-2126(16)30342-2.
  31. Cryo-EM structure of a CD4-bound open HIV-1 envelope trimer reveals structural rearrangements of the gp120 V1V2 loop. Wang H, Cohen AA, Galimidi RP, Gristick HB, Jensen GJ, Bjorkman PJ. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7151-E7158.
  32. Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit. Liu Z, Gutierrez-Vargas C, Wei J, Grassucci RA, Ramesh M, Espina N, Sun M, Tutuncuoglu B, Madison-Antenucci S, Woolford JL Jr, Tong L, Frank J. Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12174-12179.
  33. Atomic model for the membrane-embedded VO motor of a eukaryotic V-ATPase. Mazhab-Jafari MT, Rohou A, Schmidt C, Bueler SA, Benlekbir S, Robinson CV, Rubinstein JL. Nature. 2016 Oct 24:1-5. doi: 10.1038
  34. Determination of the ribosome structure to a resolution of 2.5 Å by single-particle cryo-EM. Liu Z, Gutierrez-Vargas C, Wei J, Grassucci RA, Espina N, Madison-Antenucci S, Tong L, Frank J. Protein Sci. 2016 Oct 17. doi: 10.1002/pro.3068.
  35. DNA Targeting by a Minimal CRISPR RNA-Guided Cascade. Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA. Mol Cell. 2016 Sep 1;63(5):840-51.
  36. Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation. Hussain T, Llácer JL, Wimberly BT, Kieft JS, Ramakrishnan V. Cell. 2016 Sep 22;167(1):133-144.e13.
  37. Design of a hyperstable 60-subunit protein icosahedron. Hsia Y, Bale JB, Gonen S, Shi D, Sheffler W, Fong KK, Nattermann U, Xu C, Huang PS, Ravichandran R, Yi S, Davis TN, Gonen T, King NP, Baker D. Nature. 2016 Jul 7;535(7610):136-9.
  38. Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM. Briegel A, Ortega DR, Mann P, Kjær A, Ringgaard S, Jensen GJ., Proc Natl Acad Sci U S A. 2016;113(37):10412-7
  39. Ribosome•RelA structures reveal the mechanism of stringent response activation. Loveland AB, Bah E, Madireddy R, Zhang Y, Brilot AF, Grigorieff N, Korostelev AA. Elife. 2016;5. pii: e17029.
  40. Architecture of fully occupied GluA2 AMPA receptor-TARP complex elucidated by cryo-EM. Zhao Y, Chen S, Yoshioka C, Baconguis I, Gouaux E., Nature. 2016 doi: 10.1038/nature18961.
  41. Elucidation of AMPA receptor-stargazin complexes by cryo-electron microscopy. Twomey EC, Yelshanskaya MV, Grassucci RA, Frank J, Sobolevsky AI., Science. 2016, 353(6294):83-6.
  42. Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage. Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA., Science. 2016, 351:867-71.
  43. Mechanism of NMDA Receptor Inhibition and Activation. Zhu S, Stein RA, Yoshioka C, Lee CH, Goehring A, Mchaourab HS, Gouaux E., Cell. 2016, 165:704-14.
  44. Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome. Abeyrathne PD, Koh CS, Grant T, Grigorieff N, Korostelev AA, Elife. 2016, 5. pii: e14874.
  45. Activation of NMDA receptors and the mechanism of inhibition by ifenprodil. Tajima N, Karakas E, Grant T, Simorowski N, Diaz-Avalos R, Grigorieff N, Furukawa H, Nature, 2016,  doi: 10.1038/nature17679
  46. Architecture of the type IVa pilus machine. Chang YW, Rettberg LA, Treuner-Lange A, Iwasa J, Søgaard-Andersen L, Jensen GJ. Science. 2016, 351(6278): aad2001.
  47. Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation. Yuan Z, Bai L, Sun J, Georgescu R, Liu J, O'Donnell ME, Li H; Nature Structural and Molecular Biology, 2016, 23:217-24
  48. A mechanism of viral immune evasion revealed by cryo-EM analysis of the TAP transporter. Oldham ML, Hite RK, Steffen AM, Damko E, Li Z, Walz T, Chen J; Nature, 2016, 529:537-40.
  49. Cryo-electron microscopy structure of the Slo2.2 Na+-activated K+ channel. Hite RK, Yuan P, Li Z, Hsuing Y, Walz T, MacKinnon R; Nature, 2015, 527:198-203.
  50. Glycine receptor mechanism elucidated by electron cryo-microscopy. Du J, Lü W, Wu S, Cheng Y, Gouaux E; Nature, 2015, 526:224-9.
  51. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning. Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA; Science, 2015,348:581-5.
  52. Activation of GTP hydrolysis in mRNA-tRNA translocation by elongation factor G. Li W, Liu Z, Koripella RK, Langlois R, Sanyal S, Frank J; Science Advances, 2015, 1(4). pii: e1500169.
  53. Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces. Gonen S, DiMaio F, Gonen T, Baker D; Science, 2015, 348:1365-8.
  54. Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6. Grant T, Grigorieff N; Elife, 2015, 4:e06980.
  55. Electron cryotomography studies of maturing HIV-1 particles reveal the assembly pathway of the viral core.  Woodward CL, Cheng SN, Jensen GJ; J Virol, 2015,89:1267-77.
  56. Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures. Shikuma NJ, Pilhofer M, Weiss GL, Hadfield MG, Jensen GJ, Newman DK; Science, 2014, 343:529-33.
  57. Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling. Briegel A, Ladinsky MS, Oikonomou C, Jones CW, Harris MJ, Fowler DJ, Chang YW, Thompson LK, Armitage JP, Jensen GJ; Elife, 2014, 3:e02151.
  58. Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center. Koh CS, Brilot AF, Grigorieff N, Korostelev AA; Proc Natl Acad Sci U S A, 2014, 111(25):9139-44.
  59. Structures of yeast 80S ribosome-tRNA complexes in the rotated and nonrotated conformations. Svidritskiy E, Brilot AF, Koh CS, Grigorieff N, Korostelev AA; Structure, 2014 Aug 5;22(8):1210-8.
  60. Structural basis for the prion-like MAVS filaments in antiviral innate immunity. Xu H, He X, Zheng H, Huang LJ, Hou F, Yu Z, de la Cruz MJ, Borkowski B, Zhang X, Chen ZJ, Jiang QX; Elife, 2014,3:e01489.
  61. New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography. Briegel A, Wong ML, Hodges HL, Oikonomou CM, Piasta KN, Harris MJ, Fowler DJ, Thompson LK, Falke JJ, Kiessling LL, Jensen GJ; Biochemistry, 2014, 53(10):1575-85.
  62. Structure of the ribosome with elongation factor G trapped in the pretranslocation state. Brilot AF, Korostelev AA, Ermolenko DN, Grigorieff N; Proc Natl Acad Sci U S A, 2013, 110(52):20994-9.