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jGCaMP7

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jGCaMP7

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jGCaMP7 Current Generation Fluorescent Calcium Indicators

Fluorescent calcium indicators are an important tool used to detect and measure calcium, an essential regulator of cellular signaling. These small molecule or protein indicators may be used to measure the activity of G-protein coupled receptors (GPCR) and ion channels, proteins commonly targeted by pharmaceuticals. Moreover, calcium indicators can be used to image and measure changes in Ca2+ concentrations associated with neural activity.

To date, the choice of calcium indicators has required a compromise based on experimental goals. Small-molecule synthetic dyes are the most sensitive and rapid indicators, but these dyes require invasive chemical or physical delivery methods, are difficult to target to specific cell types and subcellular locations, and do not persist when used in vivo. Conversely, genetically encoded calcium indicators (GECIs) can be targeted to specific cell types and imaged in a relatively non-invasive manner over long time scales, but suffer from limited sensitivity and slower kinetics.

Janelia scientists have developed a new generation of GECIs that overcome these barriers, displaying sensitivity and kinetics similar to synthetic dyes. The jGCaMP7 family of GECIs is a collection of ultrasensitive, green fluorescent indicator proteins that enable reliable detection of single action potential responses in vivo and facilitate the measurement of synaptic calcium signals. Variants with improved kinetic response will be useful for studying high frequency neuronal activity, offering the fastest observed kinetics of any protein sensor for cytoplasmic Ca2+.

Reagent availability:

jGCaMP7 DNA constructs, AAV particles, and Drosophila with jGCaMP7 variants were deposited for distribution at Addgene (http://www.addgene.org), the University of Pennsylvania Vector Core (http://www.med.upenn.edu/gtp/vectorcore) and the Bloomington Drosophila Stock Center (http://flystocks.bio.indiana.edu), respectively. See the lists below.

Gene sequences were deposited at NCBI GenBank with accession codes: jGCaMP7s MK749391, jGCaMP7f MK749392, jGCaMP7b MK749393, and jGCaMP7c MK749394.

Advantages:

  • Sensitivity, dynamic range, and kinetics that can exceed synthetic indicators and show significant improvement over existing GECIs
  • Several jGCaMP7 variants to select from with overall brightness, rise/decay kinetics, and calcium affinity tuned for diverse applications

Applications:

  • Neuroscience research using in vitro or in vivo models
  • Long-term functional imaging of neuronal activity and correlation with animal behavior
  • Screening for G-protein coupled receptor (GPCR) or ion channel agonists and antagonists

Patent Status:

Issued U.S. Patents:  8,629,2569,518,980, 9,488,642, 9,945,844

Pending Patents Applications: US16/198,166, PCT/US2018/062244

Opportunity:

  • Obtain the plasmid from Addgene for Non-Profit Research. 
  • Commercial Licenses are available for internal research or sale. 

For inquiries, please reference:

jGCaMP7, Janelia 2018-002

 

Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

pCMV-jGCaMP7s

improved SNR, slow kinetics

104463

Dana et al., 2019

pCMV-jGCaMP7f

improved SNR, fast kinetics

104483

Dana et al., 2019

pCMV-jGCaMP7b

improved SNR, higher baseline fluorescence

104484

Dana et al., 2019

pCMV-jGCaMP7c very low baseline fluorescence 105320 Dana et al., 2019
pAAV-syn-jGCaMP7s-WPRE improved SNR, slow kinetics 104487 Dana et al., 2019
pAAV-syn-jGCaMP7f-WPRE improved SNR, fast kinetics 104488 Dana et al., 2019
pAAV-syn-jGCaMP7b-WPRE improved SNR, higher baseline fluorescence 104489 Dana et al., 2019
pAAV-syn-jGCaMP7c-WPRE very low baseline fluorescence 105321 Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7s-WPRE improved SNR, slow kinetics, Cre-dependent expression 104491 Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7f-WPRE improved SNR, fast kinetics, Cre-dependent expression 104492 Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7b-WPRE improved SNR, higher baseline fluorescence, Cre-dependent expression 104493 Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7c-WPRE very low baseline fluorescence, Cre-dependent expression 105322 Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7s-WPRE improved SNR, slow kinetics, Cre-dependent expression 104495 Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7f-WPRE improved SNR, fast kinetics, Cre-dependent expression 104496 Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7b-WPRE improved SNR, higher baseline fluorescence, Cre-dependent expression 104497 Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7c-WPRE very low baseline fluorescence, Cre-dependent expression 105323 Dana et al., 2019

 

Viruses available from  Addgene:

Name Addgene Catalog Number Reference

AAV1-Syn-jGCaMP7s-WPRE-SV40

In production

Dana et al., 2019

AAV1-Syn-jGCaMP7f-WPRE-SV40

In production

Dana et al., 2019

AAV1-Syn-jGCaMP7b-WPRE-SV40

In production

Dana et al., 2019

AAV1-Syn-jGCaMP7c-WPRE-SV40

In production

Dana et al., 2019

AAV1-Syn-FLEX-jGCaMP7s-WPRE-SV40

In production

Dana et al., 2019

AAV1-Syn-FLEX-jGCaMP7f-WPRE-SV40

In production

Dana et al., 2019

AAV1-Syn-FLEX-jGCaMP7b-WPRE-SV40

In production

Dana et al., 2019

AAV1-Syn-FLEX-jGCaMP7c-WPRE-SV40

In production

Dana et al., 2019

AAV1-CAG-FLEX-jGCaMP7s-WPRE-SV40

In production

Dana et al., 2019

AAV1-CAG-FLEX-jGCaMP7f-WPRE-SV40

In production

Dana et al., 2019

AAV1-CAG-FLEX-jGCaMP7b-WPRE-SV40 In production Dana et al., 2019
AAV1-CAG-FLEX-jGCaMP7c-WPRE-SV40 In production Dana et al., 2019

 

Flies available from Bloomington Stock Center:

Line name Insertion Site Bloomington Stock Number Reference Chr
P{20XUAS-IVS-Syn21-op1-jGCaMP7s-p10} su(Hw)attP5 80905 Dana et al., 2019 2
P{20XUAS-IVS-Syn21-op1-jGCaMP7f-p10} su(Hw)attP5 80906 Dana et al., 2019 2
P{20XUAS-IVS-Syn21-op1-jGCaMP7b-p10} su(Hw)attP5 80907 Dana et al., 2019 2
P{20XUAS-IVS-Syn21-op1-jGCaMP7c-p10} su(Hw)attP5 80908 Dana et al., 2019 2
P{20XUAS-IVS-Syn21-op1-jGCaMP7s-p10} VK00005 79032 Dana et al., 2019 3
P{20XUAS-IVS-Syn21-op1-jGCaMP7f-p10} VK00005 79031 Dana et al., 2019 3
P{20XUAS-IVS-Syn21-op1-jGCaMP7b-p10} VK00005 79029 Dana et al., 2019 3
P{20XUAS-IVS-Syn21-op1-jGCaMP7c-p10} VK00005 79030 Dana et al., 2019 3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7s-p10} su(Hw)attP8 80909 Dana et al., 2019 X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7f-p10} su(Hw)attP8 80910 Dana et al., 2019 X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7b-p10} su(Hw)attP8 80911 Dana et al., 2019 X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7c-p10} su(Hw)attP8 80912 Dana et al., 2019 X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7s-p10} VK00005

80913

Dana et al., 2019 3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7f-p10} VK00005 80914 Dana et al., 2019 3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7b-p10} VK00005

80915

Dana et al., 2019 3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7c-p10} VK00005 80916 Dana et al., 2019 3

 

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jGCaMP7 performance
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Contact

Michael Perham
Director, Innovations and External Relations
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