OIG ABG

"Intravital Imaging of Osteoclast"

• Intravital imaging as way to discover new biology
• Developed method to image osteoclast dynamics in long bone
• Discovered osteoclast recycling as new cell fate
• Cross-validated with flow cytometry and single cell RNA sequencing
• Biological significance confirmed by role of osteomorph genes in bone formation, structure and function

Reference:

• McDonald et al. Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption. Cell. in press.

"Workshop on Computational Microscopy Image Reconstruction"

• Computational imaging and the specific challenges imposed by fluorescence microscopy
• An introduction to Scanned Line Angular Projection (SLAP) microscopy
• Richardson-Lucy image reconstruction with and without sample priors

Reference: 

• Kilohertz frame-rate two-photon tomography

"Miniaturized imaging probes: Bringing optics deep inside the body"

• We describe miniaturized fiber-optic probes for optical coherence tomography imaging deep within the body.
• Demonstrate the use of imaging needles in brain surgery.
• Demonstrate 3D printed intravascular probes to image atherosclerotic plaques in heart disease.

References:

• https://doi.org/10.1038/s41377-020-00365-w
• https://doi.org/10.1126/sciadv.aav4992

"Single-Molecule Localization Microscopy (SMLM): principles and recent advancements"

• Basic principles and history of SMLM
• How to build a 2D SMLM
• How to build a 3D SMLM (emphasis on iPALM)
• How to analyze and quantify SMLM data
• What’s next?

Reference: 

• 4Pi-RESOLFT nanoscopy

"New ways to assess platelet function: Imaging thrombus formation in molecular detail"

• Proteins on the surface of blood platelets coordinate the key initiating steps in thrombus (blood clot) formation.
• Thrombotic events are central to the initiation and prolongation of cardiovascular disease and none of the current anti-thrombotic therapies target existing blood clots.

References:

• https://onlinelibrary.wiley.com/doi/full/10.1002/adbi.201800089  
• https://www.cell.com/biophysj/fulltext/S0006-3495(21)00052-7

​"Dendritic clustering of calcium signals in behaving animals"

• How does single neuron selectivity arise with mixed inputs?
• Calcium in spines represent both input and output signals.
• What is a measure that is robust to the computational separation of input and output in calcium signals?
• Implications to dendritic clustering and other Calcium signals

Reference: 

• Functional clustering of dendritic activity during decision-making

"Insights into tumour biology and drug targeting using Intravital FLIM-FRET biosensor imaging"

• Using intravital imaging we reveal how live FLIM-FRET imaging through abdominal window can be used to assess drug targeting in live tumours deep within the abdominal cavity
• Co-assessment of stromal targeting to fine-tuned drug response and delivery to improve chemotherapy in pancreatic cancer
• Applications of FRET biosensors mice for assessing the hallmarks of cancer

References:

• https://stm.sciencemag.org/content/9/384/eaai8504.short  
• https://www.nature.com/articles/s41467-019-10968-6

"Introduction to lattice light sheet microscopy"

• Advantages of light sheet over wide field and confocal
• Comparison between Gaussian, Bessel and lattice light sheet
• Lattice theory
• Lattice light sheet microscope optical diagram
• Lattice light sheet instrumentation, data acquisition and processing
• Examples taken by lattice light sheet microscopy
• Limitations of lattice light sheet microscopy and solutions, future directions

"Live cell, 4-dimensional microscopy of the blood stage of malaria"

• Basic concepts of how to successfully set up live cell microscopy will be discussed.
• The application of lattice light sheet microscopy (LLSM) to live cell imaging of the malaria parasite, Plasmodium falciparum.
• The use of 3D SIM to complement the high resolution, diffraction-limited data obtained by LLSM.
• Approaches used for the quantitative analysis of 4-dimensional data.
• Future perspectives provided on the use of advanced microscopy in infectious disease research.

"Optogenetic mapping of neuronal interactions"

Bullets points:

• Introduction about neuronal connectivity
• The difficulty in measuring connectivity between neurons in vivo
• Optogenetic mapping of neuronal connectivity in vivo using targeted photostimulation
• What does this approach reveal about the functional organization of the motor cortex during behavior

Reference: Attractor dynamics gate cortical information flow during decision-making

"​Brillouin microscopy for biomechanical imaging"

• Overview applications of Brillouin microscopy
• Different types of Brillouin microscope: Spontaneous with VIPA, Fabry-Perot, or heterodyne; nonlinear with IBIS or SBS
• Choice of wavelength
• Mechanical properties relation to Brillouin spectra
• Limitations of Brillouin microscopy

 Reference: https://www.nature.com/articles/s41592-019-0543-3