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For High Resolution Imaging in Biological Tissues
Microscope Adaptive Optics by rear pupil masking, adaptive optics for high resolution imaging in biological tissue.
This technology advances the resolution and depth limit of light microscopy using an innovative and effective approach: adaptive optics (AO). Depth and resolution in life science imaging are limited by the inherent heterogeneity of biological specimens. These specimens give rise to optical distortions, known as aberrations that lead to the loss of signal, image fidelity and resolution. AO has been used in telescopes to address the problem of optical aberration of light beams as they propagate through our atmosphere, but it has been applied to microscopy in only a limited manner due to technical challenges. This new technology is an image based AO method that is simpler to implement than other AO methods and suitable for microscopy. This innovation comprises the use of an SLM in the optical path of a microscope and proprietary algorithms. It attains near diffraction-limited performance from a variety of biological samples with only minor modification of a light microscope.
The value of the technology has been demonstrated in two-photon microcopy, where it attained near-diffraction–limited performance in varied biological and non-biological samples exhibiting aberrations large or small and smoothly varying or abruptly changing. Work in fixed mouse cortical slices illustrates the capability to improve signal and resolution to depths of 500m. The technology is applicable to other imaging modalities, including confocal microscopy and widefield microscopy.
- Improved signal and resolution to depths of 500m
- Near diffraction limited resolution
- Requires only minor modification of the microscope
- Can be used with two photon, confocal and wide field microscopy
- Basic life science research
- Imaging of living or thick biological specimens
- In vivo calcium imaging
- Two photon microscopy
- CARS, second harmonic generation and third harmonic generation imaging
Issued U.S. Patent 8,730,573
Free for Non-Profit Research and available for Commercial License
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